REST is a hypoxia-responsive transcriptional repressor

Miguel A.S. Cavadas; Marion Mesnieres; Bianca Crifo; Mario C. Manresa; Andrew C. Selfridge; Ciara E. Keogh; Zsolt Fabian; Carsten C. Scholz; Karen A. Nolan; Liliane M.A. Rocha; Murtaza M. Tambuwala; Stuart Brown; Anita Wdowicz; Danielle Corbett; Keith J. Murphy; Catherine Godson; Eoin P. Cummins; Cormac T. Taylor; Alex Cheong

doi:10.1038/srep31355

REST is a hypoxia-responsive transcriptional repressor

Miguel A.S. Cavadas
, Marion Mesnieres
, Bianca Crifo
, Mario C. Manresa
, Andrew C. Selfridge
, Ciara E. Keogh
, Zsolt Fabian
, Carsten C. Scholz
, Karen A. Nolan
, Liliane M.A. Rocha
, Murtaza M. Tambuwala
, Stuart Brown
, Anita Wdowicz
, Danielle Corbett
, Keith J. Murphy
, Catherine Godson
, Eoin P. Cummins
, Cormac T. Taylor
, Alex Cheong^*

^*Corresponding author for this work

Universität Zürich
Universidade de Lisboa
University of Ulster
New York University School of Medicine
University College Dublin
Instituto Gulbenkian de Ciência

Research output: Contribution to journal › Article › peer-review

70 Citations (SciVal)

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Abstract

Cellular exposure to hypoxia results in altered gene expression in a range of physiologic and pathophysiologic states. Discrete cohorts of genes can be either up- or down-regulated in response to hypoxia. While the Hypoxia-Inducible Factor (HIF) is the primary driver of hypoxia-induced adaptive gene expression, less is known about the signalling mechanisms regulating hypoxiadependent
gene repression. Using RNA-seq, we demonstrate that equivalent numbers of genes are induced and repressed in human embryonic kidney (HEK293) cells. We demonstrate that nuclear localization of the Repressor Element 1-Silencing Transcription factor (REST) is induced in hypoxia and that REST is responsible for regulating approximately 20% of the hypoxia-repressed genes. Using chromatin immunoprecipitation assays we demonstrate that REST-dependent gene repression is at least in part mediated by direct binding to the promoters of target genes. Based on these data, we propose that REST is a key mediator of gene repression in hypoxia.

Original language	English
Article number	31355
Number of pages	13
Journal	Scientific Reports
Volume	6
Early online date	17 Aug 2016
DOIs	https://doi.org/10.1038/srep31355
Publication status	Published - 17 Aug 2016

Bibliographical note

© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The imageor other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Keywords

hypoxia
repressor
REST
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10.1038/srep31355Licence: CC BY 3.0

REST is a hypoxia-responsive transcriptional repressor
© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The imageor other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Final published version, 1.86 MBLicence: CC BY 3.0

Cite this

Cavadas, M. A. S., Mesnieres, M., Crifo, B., Manresa, M. C., Selfridge, A. C., Keogh, C. E., Fabian, Z., Scholz, C. C., Nolan, K. A., Rocha, L. M. A., Tambuwala, M. M., Brown, S., Wdowicz, A., Corbett, D., Murphy, K. J., Godson, C., Cummins, E. P., Taylor, C. T., & Cheong, A. (2016). REST is a hypoxia-responsive transcriptional repressor. Scientific Reports, 6, Article 31355. https://doi.org/10.1038/srep31355

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abstract = "Cellular exposure to hypoxia results in altered gene expression in a range of physiologic and pathophysiologic states. Discrete cohorts of genes can be either up- or down-regulated in response to hypoxia. While the Hypoxia-Inducible Factor (HIF) is the primary driver of hypoxia-induced adaptive gene expression, less is known about the signalling mechanisms regulating hypoxiadependent gene repression. Using RNA-seq, we demonstrate that equivalent numbers of genes are induced and repressed in human embryonic kidney (HEK293) cells. We demonstrate that nuclear localization of the Repressor Element 1-Silencing Transcription factor (REST) is induced in hypoxia and that REST is responsible for regulating approximately 20\% of the hypoxia-repressed genes. Using chromatin immunoprecipitation assays we demonstrate that REST-dependent gene repression is at least in part mediated by direct binding to the promoters of target genes. Based on these data, we propose that REST is a key mediator of gene repression in hypoxia. ",

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AU - Mesnieres, Marion

AU - Crifo, Bianca

AU - Manresa, Mario C.

AU - Selfridge, Andrew C.

AU - Keogh, Ciara E.

AU - Fabian, Zsolt

AU - Scholz, Carsten C.

AU - Nolan, Karen A.

AU - Rocha, Liliane M.A.

AU - Tambuwala, Murtaza M.

AU - Brown, Stuart

AU - Wdowicz, Anita

AU - Corbett, Danielle

AU - Murphy, Keith J.

AU - Godson, Catherine

AU - Cummins, Eoin P.

AU - Taylor, Cormac T.

AU - Cheong, Alex

N1 - © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The imageor other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PY - 2016/8/17

Y1 - 2016/8/17

N2 - Cellular exposure to hypoxia results in altered gene expression in a range of physiologic and pathophysiologic states. Discrete cohorts of genes can be either up- or down-regulated in response to hypoxia. While the Hypoxia-Inducible Factor (HIF) is the primary driver of hypoxia-induced adaptive gene expression, less is known about the signalling mechanisms regulating hypoxiadependent gene repression. Using RNA-seq, we demonstrate that equivalent numbers of genes are induced and repressed in human embryonic kidney (HEK293) cells. We demonstrate that nuclear localization of the Repressor Element 1-Silencing Transcription factor (REST) is induced in hypoxia and that REST is responsible for regulating approximately 20% of the hypoxia-repressed genes. Using chromatin immunoprecipitation assays we demonstrate that REST-dependent gene repression is at least in part mediated by direct binding to the promoters of target genes. Based on these data, we propose that REST is a key mediator of gene repression in hypoxia.

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REST is a hypoxia-responsive transcriptional repressor

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