Restriction endonuclease analysis of RAPD-PCR amplicons derived from Shiga-like toxin-producing Escherichia coli O157 isolates

Katie L. Hopkins, Anthony C. Hilton

Research output: Contribution to journalArticle

Abstract

Shiga-like toxin-producing Escherichia coli O157 isolates were characterised by random amplification of polymorphic DNA by PCR (RAPD-PCR) analysis developed to allow robust epidemiological typing of E. coli. Amplification with primer 1247 or 1290 generated a reproducible profile, but was not capable of distinguishing sufficiently between epidemiologically unrelated strains. Subsequent digestion of the amplicons with selected restriction endonucleases improved the discriminatory ability of this method for strains showing limited differentiation following RAPD-PCR analysis alone. Restriction endonuclease analysis of RAPD-PCR fragments generated from closely related strains has the potential to provide additional discriminatory information without loss of specificity.
Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalJournal of Medical Microbiology
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 2001

Fingerprint Dive into the research topics of 'Restriction endonuclease analysis of RAPD-PCR amplicons derived from Shiga-like toxin-producing Escherichia coli O157 isolates'. Together they form a unique fingerprint.

  • Cite this