Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis

N. Zarkovic, K. Zarkovic, L. Milkovic, L. Andrisic, S. Borovic, G. Waeg, D. Weber, Stuart J. Bennett, Helen R. Griffiths, T. Gune

Research output: Contribution to journalMeeting abstract

Abstract

Findings on growth regulating activities of the end-product of lipid peroxidation 4-hydroxy-2-nonenal (HNE), which acts as a “second messenger of free radicals”, overlapped with the development of antibodies specific for the aldehyde-protein adducts. These led to qualitative immunochemical determinations of the HNE presence in various pathophysiological processes and to the change of consideration of the aldehyde’s bioactivities from toxicity into cell signalling.
Moreover, findings of the HNE-protein adduct in various organs under physiological circumstances support the concept of “oxidative homeostasis”, which implies that oxidative stress and lipid peroxidation are not only pathological but also physiological processes. Reactive aldehydes, at least HNE, could play important role in oxidative homeostasis, while complementary research approaches might reveal the relevance of the aldehydic-protein adducts as major biomarkers of oxidative stress, lipid peroxidation and oxidative homeostasis.
Aiming to join efforts in such research activities researchers interacting through the International 4-Hydroxynonenal Club acting within the SFRR-International and through networking projects of the system of the European Cooperation in Science and Technology (COST) carried validation of the methods for lipid peroxidation and further developed the genuine 4-HNE-His ELISA founding quantitative and qualitative methods for detection of 4-HNE-His adducts as valuable tool to study oxidative stress and lipid peroxidation in cell cultures, various organs and tissues and eventually for human plasma and serum analyses [1].
Reference:
1. Weber, Daniela. Lidija, Milkovic. Measurement of HNE-protein adducts in human plasma and serum by ELISA—Comparison of two primary antibodies. Redox Biol. 2013. 226-233.
LanguageEnglish
PagesS14-S15
Number of pages2
JournalFree Radical Biology and Medicine
Volume65
Issue numberSuppl.1
DOIs
Publication statusPublished - 20 Sep 2013
EventSFRR - Europe 2013 meeting - Athens, Greece
Duration: 23 Sep 201325 Sep 2013

Fingerprint

Oxidative stress
Biomarkers
Histidine
Lipid Peroxidation
Oxidative Stress
Homeostasis
Lipids
Aldehydes
Plasma (human)
Proteins
Enzyme-Linked Immunosorbent Assay
Physiological Phenomena
Cell signaling
4-hydroxy-2-nonenal
Antibodies
Second Messenger Systems
Bioactivity
Serum
Cell culture
Research

Bibliographical note

SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.

Cite this

Zarkovic, N. ; Zarkovic, K. ; Milkovic, L. ; Andrisic, L. ; Borovic, S. ; Waeg, G. ; Weber, D. ; Bennett, Stuart J. ; Griffiths, Helen R. ; Gune, T. / Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis. In: Free Radical Biology and Medicine. 2013 ; Vol. 65, No. Suppl.1. pp. S14-S15.
@article{02800f681a9c415a96106dfb6f12ff34,
title = "Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis",
abstract = "Findings on growth regulating activities of the end-product of lipid peroxidation 4-hydroxy-2-nonenal (HNE), which acts as a “second messenger of free radicals”, overlapped with the development of antibodies specific for the aldehyde-protein adducts. These led to qualitative immunochemical determinations of the HNE presence in various pathophysiological processes and to the change of consideration of the aldehyde’s bioactivities from toxicity into cell signalling.Moreover, findings of the HNE-protein adduct in various organs under physiological circumstances support the concept of “oxidative homeostasis”, which implies that oxidative stress and lipid peroxidation are not only pathological but also physiological processes. Reactive aldehydes, at least HNE, could play important role in oxidative homeostasis, while complementary research approaches might reveal the relevance of the aldehydic-protein adducts as major biomarkers of oxidative stress, lipid peroxidation and oxidative homeostasis.Aiming to join efforts in such research activities researchers interacting through the International 4-Hydroxynonenal Club acting within the SFRR-International and through networking projects of the system of the European Cooperation in Science and Technology (COST) carried validation of the methods for lipid peroxidation and further developed the genuine 4-HNE-His ELISA founding quantitative and qualitative methods for detection of 4-HNE-His adducts as valuable tool to study oxidative stress and lipid peroxidation in cell cultures, various organs and tissues and eventually for human plasma and serum analyses [1].Reference: 1. Weber, Daniela. Lidija, Milkovic. Measurement of HNE-protein adducts in human plasma and serum by ELISA—Comparison of two primary antibodies. Redox Biol. 2013. 226-233.",
author = "N. Zarkovic and K. Zarkovic and L. Milkovic and L. Andrisic and S. Borovic and G. Waeg and D. Weber and Bennett, {Stuart J.} and Griffiths, {Helen R.} and T. Gune",
note = "SFRR - Europe 2013 Meeting {"}The new era of -omics in Free Radicals in Biology and Medicine{"}, 23 - 25 Sep 2013, Athens, Greece.",
year = "2013",
month = "9",
day = "20",
doi = "10.1016/j.freeradbiomed.2013.08.139",
language = "English",
volume = "65",
pages = "S14--S15",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "Suppl.1",

}

Zarkovic, N, Zarkovic, K, Milkovic, L, Andrisic, L, Borovic, S, Waeg, G, Weber, D, Bennett, SJ, Griffiths, HR & Gune, T 2013, 'Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis' Free Radical Biology and Medicine, vol. 65, no. Suppl.1, pp. S14-S15. https://doi.org/10.1016/j.freeradbiomed.2013.08.139

Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis. / Zarkovic, N.; Zarkovic, K.; Milkovic, L.; Andrisic, L.; Borovic, S.; Waeg, G.; Weber, D.; Bennett, Stuart J.; Griffiths, Helen R.; Gune, T.

In: Free Radical Biology and Medicine, Vol. 65, No. Suppl.1, 20.09.2013, p. S14-S15.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Revealing 4-hydroxynonenal-histidine adducts as qualitative and quantitative biomarker of oxidative stress, lipid peroxidation and oxidative homeostasis

AU - Zarkovic, N.

AU - Zarkovic, K.

AU - Milkovic, L.

AU - Andrisic, L.

AU - Borovic, S.

AU - Waeg, G.

AU - Weber, D.

AU - Bennett, Stuart J.

AU - Griffiths, Helen R.

AU - Gune, T.

N1 - SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.

PY - 2013/9/20

Y1 - 2013/9/20

N2 - Findings on growth regulating activities of the end-product of lipid peroxidation 4-hydroxy-2-nonenal (HNE), which acts as a “second messenger of free radicals”, overlapped with the development of antibodies specific for the aldehyde-protein adducts. These led to qualitative immunochemical determinations of the HNE presence in various pathophysiological processes and to the change of consideration of the aldehyde’s bioactivities from toxicity into cell signalling.Moreover, findings of the HNE-protein adduct in various organs under physiological circumstances support the concept of “oxidative homeostasis”, which implies that oxidative stress and lipid peroxidation are not only pathological but also physiological processes. Reactive aldehydes, at least HNE, could play important role in oxidative homeostasis, while complementary research approaches might reveal the relevance of the aldehydic-protein adducts as major biomarkers of oxidative stress, lipid peroxidation and oxidative homeostasis.Aiming to join efforts in such research activities researchers interacting through the International 4-Hydroxynonenal Club acting within the SFRR-International and through networking projects of the system of the European Cooperation in Science and Technology (COST) carried validation of the methods for lipid peroxidation and further developed the genuine 4-HNE-His ELISA founding quantitative and qualitative methods for detection of 4-HNE-His adducts as valuable tool to study oxidative stress and lipid peroxidation in cell cultures, various organs and tissues and eventually for human plasma and serum analyses [1].Reference: 1. Weber, Daniela. Lidija, Milkovic. Measurement of HNE-protein adducts in human plasma and serum by ELISA—Comparison of two primary antibodies. Redox Biol. 2013. 226-233.

AB - Findings on growth regulating activities of the end-product of lipid peroxidation 4-hydroxy-2-nonenal (HNE), which acts as a “second messenger of free radicals”, overlapped with the development of antibodies specific for the aldehyde-protein adducts. These led to qualitative immunochemical determinations of the HNE presence in various pathophysiological processes and to the change of consideration of the aldehyde’s bioactivities from toxicity into cell signalling.Moreover, findings of the HNE-protein adduct in various organs under physiological circumstances support the concept of “oxidative homeostasis”, which implies that oxidative stress and lipid peroxidation are not only pathological but also physiological processes. Reactive aldehydes, at least HNE, could play important role in oxidative homeostasis, while complementary research approaches might reveal the relevance of the aldehydic-protein adducts as major biomarkers of oxidative stress, lipid peroxidation and oxidative homeostasis.Aiming to join efforts in such research activities researchers interacting through the International 4-Hydroxynonenal Club acting within the SFRR-International and through networking projects of the system of the European Cooperation in Science and Technology (COST) carried validation of the methods for lipid peroxidation and further developed the genuine 4-HNE-His ELISA founding quantitative and qualitative methods for detection of 4-HNE-His adducts as valuable tool to study oxidative stress and lipid peroxidation in cell cultures, various organs and tissues and eventually for human plasma and serum analyses [1].Reference: 1. Weber, Daniela. Lidija, Milkovic. Measurement of HNE-protein adducts in human plasma and serum by ELISA—Comparison of two primary antibodies. Redox Biol. 2013. 226-233.

U2 - 10.1016/j.freeradbiomed.2013.08.139

DO - 10.1016/j.freeradbiomed.2013.08.139

M3 - Meeting abstract

VL - 65

SP - S14-S15

JO - Free Radical Biology and Medicine

T2 - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - Suppl.1

ER -