Role of β3-adrenergic receptors in the action of a tumour lipid mobilizing factor

Steve T. Russell, K. Hirai, Michael J. Tisdale*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Induction of lipolysis in murine white adipocytes, and stimulation of adenylate cyclase in adipocyte plasma membranes, by a tumour-produced lipid mobilizing factor, was attenuated by low concentrations (10-7-10-5M) of the specific β3-adrenoceptor antagonist SR59230A. Lipid mobilizing factor (250 nM) produced comparable increases in intracellular cyclic AMP in CHOKI cells transfected with the human β3-adrenoceptor to that obtained with isoprenaline (1 nM). In both cases cyclic AMP production was attenuated by SR59230A confirming that the effect is mediated through a β3-adrenoceptor. A non-linear regression analysis of binding of lipid mobilizing factor to the β3-adrenoceptor showed a high affinity binding site with a Kd value 78±45 nM and a Bmax value (282±1 fmole mg protein-1) comparable with that of other β3-adrenoceptor agonists. These results suggest that lipid mobilizing factor induces lipolysis through binding to a β3-adrenoceptor. © 2002 The Cancer Research Campaign.

Original languageEnglish
Pages (from-to)424-428
Number of pages5
JournalBritish Journal of Cancer
Volume86
Issue number3
DOIs
Publication statusPublished - Feb 2002

Bibliographical note

© 2002 The Cancer Research Campaign Creative Commons Attribution-NonCommercial-Share-Alike 3.0 licence, subject to the conditions listed at http://creativecommons.org/licences/by-nc-sa/3.0/

Keywords

  • β3-adrenoceptor
  • cachexia
  • energy metabolism
  • lipid mobilizing factor

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  • Student Theses

    The mode of action of a lipid mobilising factor in cancer cachexia

    Author: Russell, S. T., Apr 2002

    Supervisor: Tisdale, M. J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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