Role of lipid-mobilising factor (LMF) in protecting tumour cells from oxidative damage

P.M. Sanders, Michael J. Tisdale*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Lipid-mobilising factor (LMF) is produced by cachexia-inducing tumours and is involved in the degradation of adipose tissue, with increased oxidation of the released fatty acids through an induction of uncoupling protein (UCP) expression. Since UCP-2 is thought to be involved in the detoxification of free radicals if LMF induced UCP-2 expression in tumour cells, it might attenuate free radical toxicity. As a model system we have used MAC13 tumour cells, which do not produce LMF. Addition of LMF caused a concentration-dependent increase in UCP-2 expression, as determined by immunoblotting. This effect was attenuated by the β3 antagonist SR59230A, suggesting that it was mediated through a β3 adrenoreceptor. Co-incubation of LMF with MAC13 cells reduced the growth-inhibitory effects of bleomycin, paraquat and hydrogen peroxide, known to be free radical generators, but not chlorambucil, an alkylating agent. There was no effect of LMF alone on cellular proliferation. These results indicate that LMF antagonises the antiproliferative effect of agents working through a free radical mechanism, and may partly explain the unresponsiveness to the chemotherapy of cachexia-inducing tumours. © 2004 Cancer Research UK.

Original languageEnglish
Pages (from-to)1274-1278
Number of pages5
JournalBritish Journal of Cancer
Volume90
Issue number6
DOIs
Publication statusPublished - 22 Mar 2004

Fingerprint

Free Radicals
Neoplasms
Cachexia
Chlorambucil
Paraquat
Alkylating Agents
Bleomycin
lipid mobilizing substance
Immunoblotting
Hydrogen Peroxide
Adipose Tissue
Fatty Acids
Cell Proliferation
Drug Therapy
Growth
Research
Uncoupling Protein 2

Bibliographical note

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

To obtain permission to re-use content from this article visit RightsLink.

Keywords

  • cachexia
  • free radicals
  • lipid mobilizing factor (LMF)
  • uncoupling protein-2

Cite this

Sanders, P.M. ; Tisdale, Michael J. / Role of lipid-mobilising factor (LMF) in protecting tumour cells from oxidative damage. In: British Journal of Cancer. 2004 ; Vol. 90, No. 6. pp. 1274-1278.
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Role of lipid-mobilising factor (LMF) in protecting tumour cells from oxidative damage. / Sanders, P.M.; Tisdale, Michael J.

In: British Journal of Cancer, Vol. 90, No. 6, 22.03.2004, p. 1274-1278.

Research output: Contribution to journalArticle

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