Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids

Mohamed S. Zaibi; Claire J. Stocker; Jacqueline O'Dowd; Alison Davies; Mohamed Bellahcene; Michael A. Cawthorne; Alastair J.H. Brown; David M. Smith; Jonathan R.S. Arch

doi:10.1016/j.febslet.2010.04.027

Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids

Mohamed S. Zaibi, Claire J. Stocker, Jacqueline O'Dowd, Alison Davies, Mohamed Bellahcene, Michael A. Cawthorne, Alastair J.H. Brown, David M. Smith, Jonathan R.S. Arch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

308 Citations (SciVal)

Abstract

GPR41 is reportedly expressed in murine adipose tissue and mediates short chain fatty acid (SCFA)-stimulated leptin secretion by activating Gα_i. Here, we agree with a contradictory report in finding no expression of GPR41 in murine adipose tissue. Nevertheless, in the presence of adenosine deaminase to minimise Gα_i signalling via the adenosine A1 receptor, SCFA stimulated leptin secretion by adipocytes from wild-type but not GPR41 knockout mice. Expression of GPR43 was reduced in GPR41 knockout mice. Acetate but not butyrate stimulated leptin secretion in wild-type mesenteric adipocytes, consistent with mediation of the response by GPR43 rather than GPR41. Pertussis toxin prevented stimulation of leptin secretion by propionate in epididymal adipocytes, implicating Gα_i signalling mediated by GPR43 in SCFA-stimulated leptin secretion.

Original language	English
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2010.04.027
Publication status	Published - 3 Jun 2010

Keywords

Adipocyte
GPR41
GPR43
Leptin
Lipolysis
Short chain fatty acid

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10.1016/j.febslet.2010.04.027

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title = "Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids",

abstract = "GPR41 is reportedly expressed in murine adipose tissue and mediates short chain fatty acid (SCFA)-stimulated leptin secretion by activating Gαi. Here, we agree with a contradictory report in finding no expression of GPR41 in murine adipose tissue. Nevertheless, in the presence of adenosine deaminase to minimise Gαi signalling via the adenosine A1 receptor, SCFA stimulated leptin secretion by adipocytes from wild-type but not GPR41 knockout mice. Expression of GPR43 was reduced in GPR41 knockout mice. Acetate but not butyrate stimulated leptin secretion in wild-type mesenteric adipocytes, consistent with mediation of the response by GPR43 rather than GPR41. Pertussis toxin prevented stimulation of leptin secretion by propionate in epididymal adipocytes, implicating Gαi signalling mediated by GPR43 in SCFA-stimulated leptin secretion.",

keywords = "Adipocyte, GPR41, GPR43, Leptin, Lipolysis, Short chain fatty acid",

author = "Zaibi, {Mohamed S.} and Stocker, {Claire J.} and Jacqueline O'Dowd and Alison Davies and Mohamed Bellahcene and Cawthorne, {Michael A.} and Brown, {Alastair J.H.} and Smith, {David M.} and Arch, {Jonathan R.S.}",

year = "2010",

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doi = "10.1016/j.febslet.2010.04.027",

language = "English",

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journal = "FEBS Letters",

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T1 - Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids

AU - Zaibi, Mohamed S.

AU - Stocker, Claire J.

AU - O'Dowd, Jacqueline

AU - Davies, Alison

AU - Bellahcene, Mohamed

AU - Cawthorne, Michael A.

AU - Brown, Alastair J.H.

AU - Smith, David M.

AU - Arch, Jonathan R.S.

PY - 2010/6/3

Y1 - 2010/6/3

N2 - GPR41 is reportedly expressed in murine adipose tissue and mediates short chain fatty acid (SCFA)-stimulated leptin secretion by activating Gαi. Here, we agree with a contradictory report in finding no expression of GPR41 in murine adipose tissue. Nevertheless, in the presence of adenosine deaminase to minimise Gαi signalling via the adenosine A1 receptor, SCFA stimulated leptin secretion by adipocytes from wild-type but not GPR41 knockout mice. Expression of GPR43 was reduced in GPR41 knockout mice. Acetate but not butyrate stimulated leptin secretion in wild-type mesenteric adipocytes, consistent with mediation of the response by GPR43 rather than GPR41. Pertussis toxin prevented stimulation of leptin secretion by propionate in epididymal adipocytes, implicating Gαi signalling mediated by GPR43 in SCFA-stimulated leptin secretion.

AB - GPR41 is reportedly expressed in murine adipose tissue and mediates short chain fatty acid (SCFA)-stimulated leptin secretion by activating Gαi. Here, we agree with a contradictory report in finding no expression of GPR41 in murine adipose tissue. Nevertheless, in the presence of adenosine deaminase to minimise Gαi signalling via the adenosine A1 receptor, SCFA stimulated leptin secretion by adipocytes from wild-type but not GPR41 knockout mice. Expression of GPR43 was reduced in GPR41 knockout mice. Acetate but not butyrate stimulated leptin secretion in wild-type mesenteric adipocytes, consistent with mediation of the response by GPR43 rather than GPR41. Pertussis toxin prevented stimulation of leptin secretion by propionate in epididymal adipocytes, implicating Gαi signalling mediated by GPR43 in SCFA-stimulated leptin secretion.

KW - Adipocyte

KW - GPR41

KW - GPR43

KW - Leptin

KW - Lipolysis

KW - Short chain fatty acid

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AN - SCOPUS:77952957384

SN - 0014-5793

VL - 584

JO - FEBS Letters

JF - FEBS Letters

IS - 11

ER -

Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids

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Keywords

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