Safety and use of sputum induction in children with cystic fibrosis

Ranjan Suri, Lindsay J. Marshall, Colin Wallis, Christopher Metcalfe, Janis K. Shute, Andrew Bush

Research output: Contribution to journalArticle

Abstract

We assessed the safety and use of induced sputum (IS) in children with cystic fibrosis (CF). Forty-eight children (19 males) with CF, mean age 12.6 (range, 7.3-17.0) years and median forced expired volume in 1 sec (FEV1) 48% (range, 14-77%) predicted were recruited. Patients spontaneously expectorated sputum and then performed sputum induction by inhalation of nebulized 7% hypertonic saline. Samples were sent for bacteriological culture, and for measurement of the following inflammatory mediators: interleukin-8, myeloperoxidase, eosinophil cationic protein, and neutrophil elastase activity. FEV1 was performed before and after inhalation of hypertonic saline. There was no increase in mediator levels in IS compared to expectorated sputum (ES) samples. Only 3 patients demonstrated significant bronchoconstriction following inhalation of hypertonic saline, by the method used. From the ES samples, Pseudomonas aeruginosa was isolated in 13 patients, Staphylococcus aureus in 7 patients, Stenotrophomonas maltophilia in 1 patient, and both Pseudomonas aeruginosa and Staphylococcus aureus in 5 patients. All these organisms were found in the IS samples. However, in 2 patients whose ES grew no organisms, one patient's IS grew Pseudomonas aeruginosa, and the other patient's IS grew Staphylococcus aureus. In our study, sputum induction was safe, with no proinflammatory effect.
Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalPediatric Pulmonology
Volume35
Issue number4
DOIs
Publication statusPublished - Apr 2003

Fingerprint

Sputum
Cystic Fibrosis
Safety
Pseudomonas aeruginosa
Inhalation
Staphylococcus aureus
Stenotrophomonas maltophilia
Eosinophil Cationic Protein
Leukocyte Elastase
Bronchoconstriction
Interleukin-8
Peroxidase

Bibliographical note

Fifteenth Annual North American CF Conference, Orlando, Florida, 2001.

Keywords

  • adolescent
  • child
  • Cystic Fibrosis
  • female
  • forced expiratory volume
  • humans
  • inflammation Mediators
  • male
  • saline solution, hypertonic
  • sputum

Cite this

Suri, R., Marshall, L. J., Wallis, C., Metcalfe, C., Shute, J. K., & Bush, A. (2003). Safety and use of sputum induction in children with cystic fibrosis. Pediatric Pulmonology, 35(4), 309-313. https://doi.org/10.1002/ppul.10226
Suri, Ranjan ; Marshall, Lindsay J. ; Wallis, Colin ; Metcalfe, Christopher ; Shute, Janis K. ; Bush, Andrew. / Safety and use of sputum induction in children with cystic fibrosis. In: Pediatric Pulmonology. 2003 ; Vol. 35, No. 4. pp. 309-313.
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Suri, R, Marshall, LJ, Wallis, C, Metcalfe, C, Shute, JK & Bush, A 2003, 'Safety and use of sputum induction in children with cystic fibrosis', Pediatric Pulmonology, vol. 35, no. 4, pp. 309-313. https://doi.org/10.1002/ppul.10226

Safety and use of sputum induction in children with cystic fibrosis. / Suri, Ranjan; Marshall, Lindsay J.; Wallis, Colin; Metcalfe, Christopher; Shute, Janis K.; Bush, Andrew.

In: Pediatric Pulmonology, Vol. 35, No. 4, 04.2003, p. 309-313.

Research output: Contribution to journalArticle

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AU - Suri, Ranjan

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AU - Bush, Andrew

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Suri R, Marshall LJ, Wallis C, Metcalfe C, Shute JK, Bush A. Safety and use of sputum induction in children with cystic fibrosis. Pediatric Pulmonology. 2003 Apr;35(4):309-313. https://doi.org/10.1002/ppul.10226