Selective recognition of cyclic GMP using a fluorescence-based molecularly imprinted polymer

Nguyen Thi Kim Thanh*, Daniel L. Rathbone, David C. Billington, Nicholas A. Hartell

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Guanosine 3′,5′-cyclic monophosphate (cGMP) plays a role as a second messenger in many different biological systems. Given the ubiquitous nature of cGMP, a simple method of detecting cGMP is of interest. To that end a fluorescent polymer with recognition sites for cGMP has been prepared. Its selectivity and sensitivity were investigated and a dose-dependant decrease in fluorescence of the polymer in the presence of cGMP was observed. In contrast, virtually no effect was detected upon application of the structurally similar molecules, guanosine 5′-monophosphate (GMP) and adenosine 3′,5′-cyclic monophosphate (cAMP), thus demonstrating the high selectivity of this polymer. The association constant for the binding of cGMP to the imprinted polymer was determined in order of 3 × 10 5 M -1. A fluorescent, molecularly imprinted polymer that selectively recognises cGMP may have a useful application as a fluorescent chemosensor for cGMP detection in biological samples.

Original languageEnglish
Pages (from-to)2499-2509
Number of pages11
JournalAnalytical letters
Volume35
Issue number15
DOIs
Publication statusPublished - Dec 2002

Keywords

  • cAMP
  • cGMP
  • fluorescent monomer
  • molecular recognition
  • molecularly imprinted polymer (MIP)

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