Selectivity of response in fluorescent polymers imprinted with N1-benzylidene pyridine-2-carboxamidrazones

Daniel L. Rathbone*, Ye Ge

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Fluorescent polymers imprinted with various N1-benzylidene pyridine-2-carboxamidrazones were evaluated for their recognition of the original template and cross-reactivity to similar molecules. Dramatic quenching of fluorescence approaching background levels was observed for most cases where the "empty" MIP was re-exposed to its template. Molecules too large to enter the imprinted cavities gave no reduction of fluorescence. Other compounds were found to quench the fluorescence and are assumed to have entered the imprinted cavities. There is also evidence for partial responses which may give some measure of partial binding. The fluorescence response profiles of substrates containing polycyclic aromatics were found to be quite different from those containing flexible substituents. In order to make this approach more suitable for high-throughput screening a method has been validated wherein the extent of substrate-induced fluorescence quenching may be obtained without having to know how much polymer is present. © 2001 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalAnalytica Chimica Acta
Volume435
Issue number1
DOIs
Publication statusPublished - 17 May 2001

Bibliographical note

1st International Workshop on Molecularly Imprinted Polymers, Cardiff, Wales, 3-5 July 2000

Keywords

  • imprinted polymers
  • fluorescent monomer
  • recognition
  • molecular modelling
  • high-throughput screening

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