Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats

H. Rheinallt Parri, Timothy M. Gould, Vincenzo Crunelli

Research output: Contribution to journalArticle

Abstract

The rodent ventrobasal (VB) thalamus receives sensory inputs from the whiskers and projects to the cortex, from which it receives reciprocal excitatory afferents. Much is known about the properties and functional roles of these glutamatergic inputs to thalamocortical neurons in the VB, but no data are available on how these afferents can affect thalamic glial cells. In this study, we used combined electrophysiological recordings and intracellular calcium ([Ca(2+)](i)) imaging to investigate glial cell responses to synaptic afferent stimulation. VB thalamus glial cells can be divided into two groups based on their [Ca(2+)](i) and electrophysiological responses to sensory and corticothalamic stimulation. One group consists of astrocytes, which stain positively for S100B and preferentially load with SR101, have linear current-voltage relations and low input resistance, show no voltage-dependent [Ca(2+)](i) responses, but express mGluR5-dependent [Ca(2+)](i) transients following stimulation of the sensory and/or corticothalamic excitatory afferent pathways. Cells of the other glial group, by contrast, stain positively for NG2, and are characterized by high input resistance, the presence of voltage-dependent [Ca(2+)](i) elevations and voltage-gated inward currents. There were no synaptically induced [Ca(2+)](i) elevations in these cells under control conditions. These results show that thalamic glial cell responses to synaptic input exhibit different properties to those of thalamocortical neurons. As VB astrocytes can respond to synaptic stimulation and signal to neighbouring neurons, this glial cell organization may have functional implications for the processing of somatosensory information and modulation of behavioural state-dependent thalamocortical network activities.
LanguageEnglish
Pages29-40
Number of pages12
JournalEuropean Journal of Neuroscience
Volume32
Issue number1
Early online date6 Jul 2010
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Thalamus
Neuroglia
Neurons
Astrocytes
Coloring Agents
Afferent Pathways
Vibrissae
Automatic Data Processing
Rodentia
Calcium

Bibliographical note

Copyright Journal compilation © 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

Keywords

  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • afferent pathways
  • animals
  • Calcium
  • Cerebral Cortex
  • excitatory amino acid antagonists
  • neuroglia
  • neurons
  • patch-clamp techniques
  • rats
  • Wistar rats
  • metabotropic glutamate receptors
  • thalamus
  • vibrissae

Cite this

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title = "Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats",
abstract = "The rodent ventrobasal (VB) thalamus receives sensory inputs from the whiskers and projects to the cortex, from which it receives reciprocal excitatory afferents. Much is known about the properties and functional roles of these glutamatergic inputs to thalamocortical neurons in the VB, but no data are available on how these afferents can affect thalamic glial cells. In this study, we used combined electrophysiological recordings and intracellular calcium ([Ca(2+)](i)) imaging to investigate glial cell responses to synaptic afferent stimulation. VB thalamus glial cells can be divided into two groups based on their [Ca(2+)](i) and electrophysiological responses to sensory and corticothalamic stimulation. One group consists of astrocytes, which stain positively for S100B and preferentially load with SR101, have linear current-voltage relations and low input resistance, show no voltage-dependent [Ca(2+)](i) responses, but express mGluR5-dependent [Ca(2+)](i) transients following stimulation of the sensory and/or corticothalamic excitatory afferent pathways. Cells of the other glial group, by contrast, stain positively for NG2, and are characterized by high input resistance, the presence of voltage-dependent [Ca(2+)](i) elevations and voltage-gated inward currents. There were no synaptically induced [Ca(2+)](i) elevations in these cells under control conditions. These results show that thalamic glial cell responses to synaptic input exhibit different properties to those of thalamocortical neurons. As VB astrocytes can respond to synaptic stimulation and signal to neighbouring neurons, this glial cell organization may have functional implications for the processing of somatosensory information and modulation of behavioural state-dependent thalamocortical network activities.",
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Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats. / Parri, H. Rheinallt; Gould, Timothy M.; Crunelli, Vincenzo.

In: European Journal of Neuroscience, Vol. 32, No. 1, 07.2010, p. 29-40.

Research output: Contribution to journalArticle

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AU - Parri, H. Rheinallt

AU - Gould, Timothy M.

AU - Crunelli, Vincenzo

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