Setting up a bioreactor for recombinant protein production in yeast

Sarah Routledge*, Michelle Clare

*Corresponding author for this work

Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

Abstract

Scale-up from shake flasks to bioreactors allows for the more reproducible, high-yielding production of recombinant proteins in yeast. The ability to control growth conditions through real-time monitoring facilitates further optimization of the process. The setup of a 3-L stirred-tank bioreactor for such an application is described.

Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages99-113
Number of pages15
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Keywords

  • bioreactor
  • pichia pastoris
  • saccharomyces cerevisiae
  • scale-up

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Dive into the research topics of 'Setting up a bioreactor for recombinant protein production in yeast'. Together they form a unique fingerprint.
  • Large-scale production of secreted proteins in Pichia pastoris

    Bora, N., 25 Feb 2012, Recombinant protein production in yeast. Bill, R. M. (ed.). Humana Press, p. 217-235 19 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

  • Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

  • Optimising yeast as a host for recombinant protein production (review)

    Bonander, N. & Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 1-9 9 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

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