Setting up a bioreactor for recombinant protein production in yeast

Sarah Routledge; Michelle Clare

doi:10.1007/978-1-61779-770-5_10

Setting up a bioreactor for recombinant protein production in yeast

Sarah Routledge^*, Michelle Clare

^*Corresponding author for this work

School of Biosciences

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Scale-up from shake flasks to bioreactors allows for the more reproducible, high-yielding production of recombinant proteins in yeast. The ability to control growth conditions through real-time monitoring facilitates further optimization of the process. The setup of a 3-L stirred-tank bioreactor for such an application is described.

Original language	English
Title of host publication	Recombinant protein production in yeast
Subtitle of host publication	methods and protocols
Editors	Roslyn M. Bill
Publisher	Humana Press
Pages	99-113
Number of pages	15
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5_10
Publication status	Published - 25 Feb 2012

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Keywords

bioreactor
pichia pastoris
saccharomyces cerevisiae
scale-up

Access to Document

10.1007/978-1-61779-770-5_10

Cite this

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Setting up a bioreactor for recombinant protein production in yeast. / Routledge, Sarah; Clare, Michelle.
Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 99-113 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

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AU - Clare, Michelle

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Setting up a bioreactor for recombinant protein production in yeast

Abstract

Publication series

Keywords

Access to Document

Other files and links

Fingerprint

Large-scale production of secreted proteins in Pichia pastoris

Optimising Pichia pastoris induction

Optimising yeast as a host for recombinant protein production (review)

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