Setting up a bioreactor for recombinant protein production in yeast

Sarah Routledge*, Michelle Clare

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Scale-up from shake flasks to bioreactors allows for the more reproducible, high-yielding production of recombinant proteins in yeast. The ability to control growth conditions through real-time monitoring facilitates further optimization of the process. The setup of a 3-L stirred-tank bioreactor for such an application is described.

Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages99-113
Number of pages15
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Fingerprint

Bioreactors
Recombinant Proteins
Yeasts
Growth

Keywords

  • bioreactor
  • pichia pastoris
  • saccharomyces cerevisiae
  • scale-up

Cite this

Routledge, S., & Clare, M. (2012). Setting up a bioreactor for recombinant protein production in yeast. In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 99-113). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_10
Routledge, Sarah ; Clare, Michelle. / Setting up a bioreactor for recombinant protein production in yeast. Recombinant protein production in yeast: methods and protocols. editor / Roslyn M. Bill. Humana Press, 2012. pp. 99-113 (Methods in molecular biology). (Springer protocols).
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Routledge, S & Clare, M 2012, Setting up a bioreactor for recombinant protein production in yeast. in RM Bill (ed.), Recombinant protein production in yeast: methods and protocols. Methods in molecular biology, vol. 866, Springer protocols, Humana Press, pp. 99-113. https://doi.org/10.1007/978-1-61779-770-5_10

Setting up a bioreactor for recombinant protein production in yeast. / Routledge, Sarah; Clare, Michelle.

Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 99-113 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

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Routledge S, Clare M. Setting up a bioreactor for recombinant protein production in yeast. In Bill RM, editor, Recombinant protein production in yeast: methods and protocols. Humana Press. 2012. p. 99-113. (Methods in molecular biology). (Springer protocols). https://doi.org/10.1007/978-1-61779-770-5_10