Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

O. Bibikova, P. Singh, A. Popov, G. Akchurin, A. Skaptsov, I. Skovorodkin, V. Khanadeev, D. Mikhalevich, M. Kinnunen, G. Akchurin, V. Bogatyrev, N. Khlebtsov, S. J. Vainio, I. Meglinski, V. Tuchin

Research output: Contribution to journalArticle

Abstract

Laser optoporation of cells by local heating of plasmonic gold nanoparticles (GNPs) was proven as a favorable delivery method of molecules into cells. The optoporation efficiency depends on the laser beam intensity and GNP properties. Here, we evaluate the membrane optoporation in vitro in terms of fluorescent dye permeability under treatment of a multi-pulsed nanosecond 1064 nm laser with a sharply-focused beam. Anisotropic GNPs, such as nanorods and nanostars, were fabricated to achieve the optimal GNP-cell interaction. Nanostars demonstrated highest optoporation efficacy with more than 80% of permeabilized cells within the illuminated area. By contrast to common laser techniques, the laser beam scanning method results in cell optoporation within a controllable programmed in advance irradiated area.

Original languageEnglish
Article number055901
JournalLaser Physics Letters
Volume14
Issue number5
DOIs
Publication statusPublished - 20 May 2017

Fingerprint

cultured cells
Gold
Cells
gold
Nanoparticles
nanoparticles
Lasers
cells
lasers
Laser beams
interactions
laser beams
Nanorods
Dyes
nanorods
Membranes
delivery
Scanning
permeability
Heating

Keywords

  • cell permeability
  • HeLa cells
  • laser beam scanning
  • nanosecond laser
  • optoporation
  • plasmon-resonant gold nanoparticles

Cite this

Bibikova, O., Singh, P., Popov, A., Akchurin, G., Skaptsov, A., Skovorodkin, I., ... Tuchin, V. (2017). Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. Laser Physics Letters, 14(5), [055901]. https://doi.org/10.1088/1612-202X/aa63ae
Bibikova, O. ; Singh, P. ; Popov, A. ; Akchurin, G. ; Skaptsov, A. ; Skovorodkin, I. ; Khanadeev, V. ; Mikhalevich, D. ; Kinnunen, M. ; Akchurin, G. ; Bogatyrev, V. ; Khlebtsov, N. ; Vainio, S. J. ; Meglinski, I. ; Tuchin, V. / Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. In: Laser Physics Letters. 2017 ; Vol. 14, No. 5.
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Bibikova, O, Singh, P, Popov, A, Akchurin, G, Skaptsov, A, Skovorodkin, I, Khanadeev, V, Mikhalevich, D, Kinnunen, M, Akchurin, G, Bogatyrev, V, Khlebtsov, N, Vainio, SJ, Meglinski, I & Tuchin, V 2017, 'Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure', Laser Physics Letters, vol. 14, no. 5, 055901. https://doi.org/10.1088/1612-202X/aa63ae

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. / Bibikova, O.; Singh, P.; Popov, A.; Akchurin, G.; Skaptsov, A.; Skovorodkin, I.; Khanadeev, V.; Mikhalevich, D.; Kinnunen, M.; Akchurin, G.; Bogatyrev, V.; Khlebtsov, N.; Vainio, S. J.; Meglinski, I.; Tuchin, V.

In: Laser Physics Letters, Vol. 14, No. 5, 055901, 20.05.2017.

Research output: Contribution to journalArticle

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AU - Bibikova, O.

AU - Singh, P.

AU - Popov, A.

AU - Akchurin, G.

AU - Skaptsov, A.

AU - Skovorodkin, I.

AU - Khanadeev, V.

AU - Mikhalevich, D.

AU - Kinnunen, M.

AU - Akchurin, G.

AU - Bogatyrev, V.

AU - Khlebtsov, N.

AU - Vainio, S. J.

AU - Meglinski, I.

AU - Tuchin, V.

PY - 2017/5/20

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Bibikova O, Singh P, Popov A, Akchurin G, Skaptsov A, Skovorodkin I et al. Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. Laser Physics Letters. 2017 May 20;14(5). 055901. https://doi.org/10.1088/1612-202X/aa63ae