Silk fibroin-polyurethane blends: physical properties and effect of silk fibroin content on viscoelasticity, biocompatibility and myoblast differentiation

Hyung-seok Park, Myoung-Seon Gong, Jeong-Hui Park, Sung-Il Moon, Ivan B Wall, Hae-Won Kim, Jae Ho Lee, Jonathan C Knowles

Research output: Contribution to journalArticlepeer-review

Abstract

As a way to modify both the physical and biological properties of a highly elastic and degradable polyurethane (PU), silk fibroin (SF) was blended with the PU at differing ratios. With increasing SF content, the tensile strength decreased as did the strain at break; the stiffness increased to around 35 MPa for the highest silk content. C2C12 (a mouse myoblast cell line) cells were used for in vitro experiments and showed significantly improved cell responses with increasing SF content. With increasing SF content the number of non-adherent cells was reduced at both 4 and 8h compared to the sample with the lowest SF content. In addition, muscle marker genes were upregulated compared to the sample containing no SF, and in particular sarcomeric actin and α-actin.

Original languageEnglish
Pages (from-to)8962-8971
Number of pages10
JournalActa Biomaterialia
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2013

Bibliographical note

Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

Keywords

  • Animals
  • Biocompatible Materials/pharmacology
  • Cell Adhesion/drug effects
  • Cell Count
  • Cell Differentiation/drug effects
  • Cell Line
  • Cell Proliferation/drug effects
  • Cell Shape/drug effects
  • Elastic Modulus/drug effects
  • Elasticity/drug effects
  • Fibroins/chemistry
  • Fluorescent Antibody Technique
  • Focal Adhesion Protein-Tyrosine Kinases/metabolism
  • Gene Expression Regulation/drug effects
  • Materials Testing
  • Membranes, Artificial
  • Mice
  • Muscle Development/drug effects
  • Myoblasts/cytology
  • Polyurethanes/chemical synthesis
  • RNA, Messenger/genetics
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Tensile Strength/drug effects
  • Thermogravimetry
  • Viscosity/drug effects

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