Simple and effective administration and visualization of microparticles in the circulatory system of small fishes using kidney injection

Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Dmitry Karnaukhov, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The systemic administration of micro-size particles into a living organism can be applied for vasculature visualization, drug and vaccine delivery, implantation of transgenic cells and tiny optical sensors. However, intravenous microinjections into small animals, which are mostly used in biological and veterinary laboratories, are very difficult and require trained personnel. Herein, we demonstrate a robust and efficient method for the introduction of microparticles into the circulatory system of adult zebrafish (Danio rerio) by injection into the fish kidney. To visualize the introduced microparticles in the vasculature, we propose a simple intravital imaging technique in fish gills. In vivo monitoring of the zebrafish blood pH was accomplished using an injected microencapsulated fluorescent probe, SNARF-1, to demonstrate one of the possible applications of the described technique. This article provides a detailed description of the encapsulation of pH-sensitive dye and demonstrates the principles of the quick injection and visualization of the obtained microcapsules for in vivo recording of the fluorescent signal. The proposed method of injection is characterized by a low mortality rate (0-20%) and high efficiency (70-90% success), and it is easy to institute using commonly available equipment. All described procedures can be performed on other small fish species, such as guppies and medaka.

Original languageEnglish
Article numbere57491
JournalJournal of Visualized Experiments
Volume2018
Issue number136
DOIs
Publication statusPublished - 17 Jun 2018

Keywords

  • Biology
  • Danio rerio
  • In vivo diagnostics
  • Injection
  • Issue 136
  • Layer-by-layer assembly
  • Microencapsulated biomarkers
  • Microparticles
  • Optical sensor
  • Remote physiological measurements
  • Systemic administration

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