Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID)

Richard A. Armstrong, Marla Gearing, Eileen H. Bigio, Felix F. Cruz-Sanchez, Charles Duyckaerts, Ian R.A. Mackenzie, Robert H Perry, Kari Skullerud, Hideaki Yokoo, Nigel J. Cairns

Research output: Contribution to journalArticle

Abstract

Neuronal intermediate filament inclusion disease (NIFID), a rare form of frontotemporal lobar degeneration (FTLD), is characterized neuropathologically by focal atrophy of the frontal and temporal lobes, neuronal loss, gliosis, and neuronal cytoplasmic inclusions (NCI) containing epitopes of ubiquitin and neuronal intermediate filament (IF) proteins. Recently, the 'fused in sarcoma' (FUS) protein (encoded by the FUS gene) has been shown to be a component of the inclusions of NIFID. To further characterize FUS proteinopathy in NIFID, we studied the spatial patterns of the FUS-immunoreactive NCI in frontal and temporal cortex of 10 cases. In the cerebral cortex, sectors CA1/2 of the hippocampus, and the dentate gyrus (DG), the FUS-immunoreactive NCI were frequently clustered and the clusters were regularly distributed parallel to the tissue boundary. In a proportion of cortical gyri, cluster size of the NCI approximated to those of the columns of cells was associated with the cortico-cortical projections. There were no significant differences in the frequency of different types of spatial patterns with disease duration or disease stage. Clusters of NCI in the upper and lower cortex were significantly larger using FUS compared with phosphorylated, neurofilament heavy polypeptide (NEFH) or a-internexin (INA) immunohistochemistry (IHC). We concluded: (1) FUS-immunoreactive NCI exhibit similar spatial patterns to analogous inclusions in the tauopathies and synucleinopathies, (2) clusters of FUS-immunoreactive NCI are larger than those revealed by NEFH or ???, and (3) the spatial patterns of the FUS-immunoreactive NCI suggest the degeneration of the cortico-cortical projections in NIFID.
LanguageEnglish
Pages1651-1657
Number of pages7
JournalJournal of Neural Transmission
Volume118
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Intermediate Filaments
Inclusion Bodies
Cytomegalovirus Infections
Sarcoma
Frontal Lobe
Temporal Lobe
Frontotemporal Lobar Degeneration
Tauopathies
Intermediate Filament Proteins
Peptides
Gliosis
Dentate Gyrus
Ubiquitin
Cerebral Cortex
Atrophy
Epitopes
Hippocampus
Immunohistochemistry

Keywords

  • neurofilament intermediate filament inclusion disease
  • NIFID
  • fused in sarcoma
  • FUS
  • neuronal cytoplasmic inclusions
  • NCI
  • spatial pattern
  • cortico-cortical projections

Cite this

Armstrong, R. A., Gearing, M., Bigio, E. H., Cruz-Sanchez, F. F., Duyckaerts, C., Mackenzie, I. R. A., ... Cairns, N. J. (2011). Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID). Journal of Neural Transmission, 118(11), 1651-1657. https://doi.org/10.1007/s00702-011-0690-x
Armstrong, Richard A. ; Gearing, Marla ; Bigio, Eileen H. ; Cruz-Sanchez, Felix F. ; Duyckaerts, Charles ; Mackenzie, Ian R.A. ; Perry, Robert H ; Skullerud, Kari ; Yokoo, Hideaki ; Cairns, Nigel J. / Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID). In: Journal of Neural Transmission. 2011 ; Vol. 118, No. 11. pp. 1651-1657.
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Armstrong, RA, Gearing, M, Bigio, EH, Cruz-Sanchez, FF, Duyckaerts, C, Mackenzie, IRA, Perry, RH, Skullerud, K, Yokoo, H & Cairns, NJ 2011, 'Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID)' Journal of Neural Transmission, vol. 118, no. 11, pp. 1651-1657. https://doi.org/10.1007/s00702-011-0690-x

Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID). / Armstrong, Richard A.; Gearing, Marla; Bigio, Eileen H.; Cruz-Sanchez, Felix F.; Duyckaerts, Charles; Mackenzie, Ian R.A.; Perry, Robert H; Skullerud, Kari; Yokoo, Hideaki; Cairns, Nigel J.

In: Journal of Neural Transmission, Vol. 118, No. 11, 11.2011, p. 1651-1657.

Research output: Contribution to journalArticle

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T1 - Spatial patterns of FUS-immunoreactive neuronal cytoplasmic inclusions (NCI) in neuronal intermediate filament inclusion disease (NIFID)

AU - Armstrong, Richard A.

AU - Gearing, Marla

AU - Bigio, Eileen H.

AU - Cruz-Sanchez, Felix F.

AU - Duyckaerts, Charles

AU - Mackenzie, Ian R.A.

AU - Perry, Robert H

AU - Skullerud, Kari

AU - Yokoo, Hideaki

AU - Cairns, Nigel J.

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KW - spatial pattern

KW - cortico-cortical projections

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