Stabilising cysteinyl thiol oxidation and nitrosation for proteomic analysis

Shibani Ratnayake, Irundika H.K. Dias, Eric Lattmann, Helen R. Griffiths

Research output: Contribution to journalArticle

Abstract

Oxidation and S-nitrosylation of cysteinyl thiols (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H), sulfonic acids (Cys-SO3H), disulphides and S-nitrosothiols are suggested as important post-translational modifications that can activate or deactivate the function of many proteins. Non-enzymatic post-translational modifications to cysteinyl thiols have been implicated in a wide variety of physiological and pathophysiological states but have been difficult to monitor in a physiological setting because of a lack of experimental tools. The purpose of this review is to bring together the approaches that have been developed for stably trapping cysteine either in its reduced or oxidised forms for enrichment and or subsequent mass spectrometric analysis. These tools are providing insight into potential targets for post-translational modifications to cysteine modification in vivo. This article is part of a Special Issue entitled: Special Issue: Posttranslational Protein modifications in biology and Medicine. © 2013.

Original languageEnglish
Pages (from-to)160-170
Number of pages11
JournalJournal of Proteomics
Volume92
Early online date21 Jun 2013
DOIs
Publication statusPublished - 30 Oct 2013

Fingerprint

Nitrosation
Post Translational Protein Processing
Sulfhydryl Compounds
Proteomics
Cysteine
S-Nitrosothiols
Oxidation
Sulfonic Acids
Disulfides
Medicine
Proteins
cysteinesulfenic acid

Keywords

  • dimedone
  • gel electrophoresis
  • ICAT
  • nitrosothiol
  • sulfenic acid

Cite this

Ratnayake, Shibani ; Dias, Irundika H.K. ; Lattmann, Eric ; Griffiths, Helen R. / Stabilising cysteinyl thiol oxidation and nitrosation for proteomic analysis. In: Journal of Proteomics. 2013 ; Vol. 92. pp. 160-170.
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Stabilising cysteinyl thiol oxidation and nitrosation for proteomic analysis. / Ratnayake, Shibani; Dias, Irundika H.K.; Lattmann, Eric; Griffiths, Helen R.

In: Journal of Proteomics, Vol. 92, 30.10.2013, p. 160-170.

Research output: Contribution to journalArticle

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