Structural modification of styrene Maleic anhydride copolymers for plant bioactive compound extraction

Patchara Punyamoonwongsa, Pattana Kakumyan, Natsaran Saichana, Brian Tighe

Research output: Chapter in Book/Report/Conference proceedingConference publication

Abstract

Ability of poly(styrene-alt-maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α-helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive compounds are recovered from plant tissues for different reasons. One of the most important reasons is due to the increased demand in nutraceuticals market and modern therapeutics. Despite this, aqueous-based extraction of these compounds has been reported to give low extraction yield. A development of new green extraction protocol is still a challenging task for all researchers nowadays. This study demonstrated, for the first time, possible use of PSMA as a lysis agent for plant bioactive compound extraction. To enhance its membrane affinity at physiological pH, the polymer was esterified with methanol. Both PSMA and its derivative (ePSMA) were characterized in terms of their membrane binding affinity through a combined use of both surface characterization and physical techniques. Analysis of the ternary phase diagrams suggested that ePSMA could facilitate stronger hydrophobically-driven interactions with the lipid. This was convinced by the reduced critical PSMA/lipid mass ratio from 7:1 (PSMA) to 1:1 (ePSMA), as observed in the ternary phase diagrams. Last but not the least, the crude extracts of Coffea robusta leaves obtained from ePSMA-based extraction showed a total phenolic content of 20.32±0.75 mg/g sample, significantly higher than that from the PSMA-(14.24±1.27 mg/g sample) and aqueous-based (16.33±1.03 mg/g sample) extractions. A structural manipulation of PSMA is thus a key to tailor its membrane solubilization and so, the extraction efficacy of bioactive compounds from plant cells.

Original languageEnglish
Title of host publicationMaterials Science and Technology X
EditorsPeerapong Pinwanich, Akrapol Soisungval
PublisherTrans Tech Publications
Pages351-357
Number of pages7
ISBN (Print)9783035714296
DOIs
Publication statusPublished - 1 Apr 2019
Event10th International Conference on Materials Science and Technology, MSAT 2018 - Bangkok, Thailand
Duration: 6 Sep 20187 Sep 2018

Publication series

NameKey Engineering Materials
Volume798 KEM
ISSN (Print)1013-9826

Conference

Conference10th International Conference on Materials Science and Technology, MSAT 2018
CountryThailand
CityBangkok
Period6/09/187/09/18

Keywords

  • Cell lysis
  • Membrane solubilization
  • Plant extraction
  • Styrene maleic anhydride

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