TY - GEN
T1 - Structural modification of styrene Maleic anhydride copolymers for plant bioactive compound extraction
AU - Punyamoonwongsa, Patchara
AU - Kakumyan, Pattana
AU - Saichana, Natsaran
AU - Tighe, Brian
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Ability of poly(styrene-alt-maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α-helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive compounds are recovered from plant tissues for different reasons. One of the most important reasons is due to the increased demand in nutraceuticals market and modern therapeutics. Despite this, aqueous-based extraction of these compounds has been reported to give low extraction yield. A development of new green extraction protocol is still a challenging task for all researchers nowadays. This study demonstrated, for the first time, possible use of PSMA as a lysis agent for plant bioactive compound extraction. To enhance its membrane affinity at physiological pH, the polymer was esterified with methanol. Both PSMA and its derivative (ePSMA) were characterized in terms of their membrane binding affinity through a combined use of both surface characterization and physical techniques. Analysis of the ternary phase diagrams suggested that ePSMA could facilitate stronger hydrophobically-driven interactions with the lipid. This was convinced by the reduced critical PSMA/lipid mass ratio from 7:1 (PSMA) to 1:1 (ePSMA), as observed in the ternary phase diagrams. Last but not the least, the crude extracts of Coffea robusta leaves obtained from ePSMA-based extraction showed a total phenolic content of 20.32±0.75 mg/g sample, significantly higher than that from the PSMA-(14.24±1.27 mg/g sample) and aqueous-based (16.33±1.03 mg/g sample) extractions. A structural manipulation of PSMA is thus a key to tailor its membrane solubilization and so, the extraction efficacy of bioactive compounds from plant cells.
AB - Ability of poly(styrene-alt-maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α-helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive compounds are recovered from plant tissues for different reasons. One of the most important reasons is due to the increased demand in nutraceuticals market and modern therapeutics. Despite this, aqueous-based extraction of these compounds has been reported to give low extraction yield. A development of new green extraction protocol is still a challenging task for all researchers nowadays. This study demonstrated, for the first time, possible use of PSMA as a lysis agent for plant bioactive compound extraction. To enhance its membrane affinity at physiological pH, the polymer was esterified with methanol. Both PSMA and its derivative (ePSMA) were characterized in terms of their membrane binding affinity through a combined use of both surface characterization and physical techniques. Analysis of the ternary phase diagrams suggested that ePSMA could facilitate stronger hydrophobically-driven interactions with the lipid. This was convinced by the reduced critical PSMA/lipid mass ratio from 7:1 (PSMA) to 1:1 (ePSMA), as observed in the ternary phase diagrams. Last but not the least, the crude extracts of Coffea robusta leaves obtained from ePSMA-based extraction showed a total phenolic content of 20.32±0.75 mg/g sample, significantly higher than that from the PSMA-(14.24±1.27 mg/g sample) and aqueous-based (16.33±1.03 mg/g sample) extractions. A structural manipulation of PSMA is thus a key to tailor its membrane solubilization and so, the extraction efficacy of bioactive compounds from plant cells.
KW - Cell lysis
KW - Membrane solubilization
KW - Plant extraction
KW - Styrene maleic anhydride
UR - http://www.scopus.com/inward/record.url?scp=85067649987&partnerID=8YFLogxK
UR - https://www.scientific.net/KEM.798.351
U2 - 10.4028/www.scientific.net/KEM.798.351
DO - 10.4028/www.scientific.net/KEM.798.351
M3 - Conference publication
AN - SCOPUS:85067649987
SN - 9783035714296
T3 - Key Engineering Materials
SP - 351
EP - 357
BT - Materials Science and Technology X
A2 - Pinwanich, Peerapong
A2 - Soisungval, Akrapol
PB - Trans Tech Publications
T2 - 10th International Conference on Materials Science and Technology, MSAT 2018
Y2 - 6 September 2018 through 7 September 2018
ER -