Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

Andrew D. Palmer, Rian Griffiths, Iain Styles, Ela Claridge, Antonio Calcagni, Josephine Bunch

Research output: Contribution to journalArticle

Abstract

Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-μm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalJournal of Mass Spectrometry
Volume47
Issue number2
DOIs
Publication statusPublished - Feb 2012

Fingerprint

sucrose
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Sugar (sucrose)
Formaldehyde
Freezing
Lipids
Mass spectrometry
Sucrose
Mass Spectrometry
mass spectroscopy
Imaging techniques
Artifacts
freezing
lipids
artifacts
acquisition
Swine
Experiments

Keywords

  • cryo-protection
  • eye
  • histology
  • imaging
  • MALDI
  • sucrose

Cite this

Palmer, A. D., Griffiths, R., Styles, I., Claridge, E., Calcagni, A., & Bunch, J. (2012). Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry. Journal of Mass Spectrometry, 47(2), 237-241. https://doi.org/10.1002/jms.2049
Palmer, Andrew D. ; Griffiths, Rian ; Styles, Iain ; Claridge, Ela ; Calcagni, Antonio ; Bunch, Josephine. / Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry. In: Journal of Mass Spectrometry. 2012 ; Vol. 47, No. 2. pp. 237-241.
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Palmer, AD, Griffiths, R, Styles, I, Claridge, E, Calcagni, A & Bunch, J 2012, 'Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry', Journal of Mass Spectrometry, vol. 47, no. 2, pp. 237-241. https://doi.org/10.1002/jms.2049

Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry. / Palmer, Andrew D.; Griffiths, Rian; Styles, Iain; Claridge, Ela; Calcagni, Antonio; Bunch, Josephine.

In: Journal of Mass Spectrometry, Vol. 47, No. 2, 02.2012, p. 237-241.

Research output: Contribution to journalArticle

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