Surface microbial consortia from Livarot, a French smear-ripened cheese

Sandra Larpin-Laborde, Muhammad Imran, Catherine Bonaïti, Nagamani Bora, Roberto Gelsomino, Stefanie Goerges, Françoise Irlinger, Michael Goodfellow, Alan C. Ward, Marc Vancanneyt, Jean Swings, Siegfried Scherer, Micheline Guéguen, Nathalie Desmasures*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The surface microflora (902 isolates) of Livarot cheeses from three dairies was investigated during ripening. Yeasts were mainly identified by Fourier transform infrared spectroscopy. Geotrichum candidum was the dominating yeast among 10 species. Bacteria were identified using Biotype 100 strips, dereplicated by repetitive extragenic palindromic PCR (rep-PCR); 156 representative strains were identified by either BOX-PCR or (GTG) 55-PCR, and when appropriate by 16S rDNA sequencing and SDS-PAGE analysis. Gram-positive bacteria accounted for 65% of the isolates and were mainly assigned to the genera Arthrobacter, Brevibacterium, Corynebacterium, and Staphylococcus. New taxa related to the genera Agrococcus and Leucobacter were found. Yeast and Gram-positive bacteria strains deliberately added as smearing agents were sometimes undetected during ripening. Thirty-two percent of the isolates were Gram-negative bacteria, which showed a high level of diversity and mainly included members of the genera Alcaligenes, Hafnia, Proteus, Pseudomonas, and Psychrobacter. Whatever the milk used (pasteurized or unpasteurized), similar levels of biodiversity were observed in the three dairies, all of which had efficient cleaning procedures and good manufacturing practices. It appears that some of the Gramnegative bacteria identified should now be regarded as potentially useful in some cheese technologies. The assessment of their positive versus negative role should be objectively examined.

Original languageEnglish
Pages (from-to)651-660
Number of pages10
JournalCanadian Journal of Microbiology
Volume57
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Microbial Consortia
Cheese
Yeasts
Gram-Positive Bacteria
Polymerase Chain Reaction
Hafnia
Psychrobacter
Brevibacterium
Geotrichum
Alcaligenes
Arthrobacter
Bacteria
Corynebacterium
Proteus
Biodiversity
Fourier Transform Infrared Spectroscopy
Ribosomal DNA
Pseudomonas
Gram-Negative Bacteria
Staphylococcus

Keywords

  • bacteria
  • diversity
  • dynamics
  • smear cheese
  • yeast

Cite this

Larpin-Laborde, S., Imran, M., Bonaïti, C., Bora, N., Gelsomino, R., Goerges, S., ... Desmasures, N. (2011). Surface microbial consortia from Livarot, a French smear-ripened cheese. Canadian Journal of Microbiology, 57(8), 651-660. https://doi.org/10.1139/w11-050
Larpin-Laborde, Sandra ; Imran, Muhammad ; Bonaïti, Catherine ; Bora, Nagamani ; Gelsomino, Roberto ; Goerges, Stefanie ; Irlinger, Françoise ; Goodfellow, Michael ; Ward, Alan C. ; Vancanneyt, Marc ; Swings, Jean ; Scherer, Siegfried ; Guéguen, Micheline ; Desmasures, Nathalie. / Surface microbial consortia from Livarot, a French smear-ripened cheese. In: Canadian Journal of Microbiology. 2011 ; Vol. 57, No. 8. pp. 651-660.
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Larpin-Laborde, S, Imran, M, Bonaïti, C, Bora, N, Gelsomino, R, Goerges, S, Irlinger, F, Goodfellow, M, Ward, AC, Vancanneyt, M, Swings, J, Scherer, S, Guéguen, M & Desmasures, N 2011, 'Surface microbial consortia from Livarot, a French smear-ripened cheese', Canadian Journal of Microbiology, vol. 57, no. 8, pp. 651-660. https://doi.org/10.1139/w11-050

Surface microbial consortia from Livarot, a French smear-ripened cheese. / Larpin-Laborde, Sandra; Imran, Muhammad; Bonaïti, Catherine; Bora, Nagamani; Gelsomino, Roberto; Goerges, Stefanie; Irlinger, Françoise; Goodfellow, Michael; Ward, Alan C.; Vancanneyt, Marc; Swings, Jean; Scherer, Siegfried; Guéguen, Micheline; Desmasures, Nathalie.

In: Canadian Journal of Microbiology, Vol. 57, No. 8, 08.2011, p. 651-660.

Research output: Contribution to journalArticle

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AU - Larpin-Laborde, Sandra

AU - Imran, Muhammad

AU - Bonaïti, Catherine

AU - Bora, Nagamani

AU - Gelsomino, Roberto

AU - Goerges, Stefanie

AU - Irlinger, Françoise

AU - Goodfellow, Michael

AU - Ward, Alan C.

AU - Vancanneyt, Marc

AU - Swings, Jean

AU - Scherer, Siegfried

AU - Guéguen, Micheline

AU - Desmasures, Nathalie

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Larpin-Laborde S, Imran M, Bonaïti C, Bora N, Gelsomino R, Goerges S et al. Surface microbial consortia from Livarot, a French smear-ripened cheese. Canadian Journal of Microbiology. 2011 Aug;57(8):651-660. https://doi.org/10.1139/w11-050