TY - JOUR
T1 - Synthesis of bilayer films from regenerated cellulose nanofibers and poly(globalide) for skin tissue engineering applications
AU - Amaral, Heliane R.
AU - Wilson, James A.
AU - Amaral, Ronaldo J.F.C. do
AU - Pasçu, Irina
AU - Oliveira, Fernando C.S. de
AU - Kearney, Cathal J.
AU - Freitas, Jair C.C.
AU - Heise, Andreas
PY - 2021/1/15
Y1 - 2021/1/15
N2 - Commercial cell-based skin regenerative products are highly expensive, carry the risk of rejection and require a long cell culture period to manufacture. This work describes the synthesis of bilayer films from poly(globalide) (PGl) and regenerated cellulose nanofibers (rCNFs) and their use as a cell-free scaffold to support keratinocyte attachment and proliferation. The method is simple, eco-friendly (as the cellulose precursor is obtained from agricultural waste) and of low cost. The rCNFs were produced by acid hydrolysis and PGl was obtained via enzymatic ring-opening polymerization. The bilayer films were synthesized by layer-by-layer casting at ambient temperature. All the films showed a well-defined interface between PGl and cellulose. The produced rCNF/PGl bilayer films showed cell metabolic activity far superior in comparison with pristine PGl regarding the keratinocyte growth, which illustrates the potential use of these materials in skin tissue engineering.
AB - Commercial cell-based skin regenerative products are highly expensive, carry the risk of rejection and require a long cell culture period to manufacture. This work describes the synthesis of bilayer films from poly(globalide) (PGl) and regenerated cellulose nanofibers (rCNFs) and their use as a cell-free scaffold to support keratinocyte attachment and proliferation. The method is simple, eco-friendly (as the cellulose precursor is obtained from agricultural waste) and of low cost. The rCNFs were produced by acid hydrolysis and PGl was obtained via enzymatic ring-opening polymerization. The bilayer films were synthesized by layer-by-layer casting at ambient temperature. All the films showed a well-defined interface between PGl and cellulose. The produced rCNF/PGl bilayer films showed cell metabolic activity far superior in comparison with pristine PGl regarding the keratinocyte growth, which illustrates the potential use of these materials in skin tissue engineering.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85092687264&origin=resultslist
UR - https://www.sciencedirect.com/science/article/pii/S0144861720313746?via%3Dihub
U2 - 10.1016/j.carbpol.2020.117201
DO - 10.1016/j.carbpol.2020.117201
M3 - Article
SN - 0144-8617
VL - 252
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 117201
ER -