Tear analysis and lens-tear interactions: part II. Ocular lipids-nature and fate of meibomian gland phospholipids

Research output: Contribution to journalArticle

Abstract

Purpose: Published data indicate that the polar lipid content of human meibomian gland secretions (MGS) could be anything between 0.5% and 13% of the total lipid. The tear film phospholipid composition has not been studied in great detail and it has been understood that the relative proportions of lipids in MGS would be maintained in the tear film. The purpose of this work was to determine the concentration of phospholipids in the human tear film. Methods: Liquid chromatography mass spectrometry (LCMS) and thin layer chromatography (TLC) were used to determine the concentration of phospholipid in the tear film. Additionally, an Amplex Red phosphatidylcholine-specific phospholipase C (PLC) assay kit was used for determination of the activity of PLC in the tear film. Results: Phospholipids were not detected in any of the tested human tear samples with the low limit of detection being 1.3 µg/mL for TLC and 4 µg/mL for liquid chromatography mass spectrometry. TLC indicated that diacylglycerol (DAG) may be present in the tear film. PLC was in the tear film with an activity determined at approximately 15 mU/mL, equivalent to the removal of head groups from phosphatidylcholine at a rate of approximately 15 µM/min. Conclusions: This work shows that phospholipid was not detected in any of the tested human tear samples (above the lower limits of detection as described) and suggests the presence of DAG in the tear film. DAG is known to be at low concentrations in MGS. These observations indicate that PLC may play a role in modulating the tear film phospholipid concentration.
Original languageEnglish
Pages (from-to)325-332
Number of pages8
JournalCornea
Volume30
Issue number3
DOIs
Publication statusPublished - Mar 2011

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Meibomian Glands
Tears
Lenses
Phospholipids
Lipids
Diglycerides
Type C Phospholipases
Thin Layer Chromatography
Liquid Chromatography
Limit of Detection
Mass Spectrometry
Phosphatidylcholines

Keywords

  • polar lipid
  • human meibomian gland secretions
  • phospholipid composition
  • liquid chromatography mass spectrometry
  • thin layer chromatography
  • Amplex Red phosphatidylcholine-specific phospholipase C
  • diacylglycerol

Cite this

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title = "Tear analysis and lens-tear interactions: part II. Ocular lipids-nature and fate of meibomian gland phospholipids",
abstract = "Purpose: Published data indicate that the polar lipid content of human meibomian gland secretions (MGS) could be anything between 0.5{\%} and 13{\%} of the total lipid. The tear film phospholipid composition has not been studied in great detail and it has been understood that the relative proportions of lipids in MGS would be maintained in the tear film. The purpose of this work was to determine the concentration of phospholipids in the human tear film. Methods: Liquid chromatography mass spectrometry (LCMS) and thin layer chromatography (TLC) were used to determine the concentration of phospholipid in the tear film. Additionally, an Amplex Red phosphatidylcholine-specific phospholipase C (PLC) assay kit was used for determination of the activity of PLC in the tear film. Results: Phospholipids were not detected in any of the tested human tear samples with the low limit of detection being 1.3 µg/mL for TLC and 4 µg/mL for liquid chromatography mass spectrometry. TLC indicated that diacylglycerol (DAG) may be present in the tear film. PLC was in the tear film with an activity determined at approximately 15 mU/mL, equivalent to the removal of head groups from phosphatidylcholine at a rate of approximately 15 µM/min. Conclusions: This work shows that phospholipid was not detected in any of the tested human tear samples (above the lower limits of detection as described) and suggests the presence of DAG in the tear film. DAG is known to be at low concentrations in MGS. These observations indicate that PLC may play a role in modulating the tear film phospholipid concentration.",
keywords = "polar lipid, human meibomian gland secretions, phospholipid composition, liquid chromatography mass spectrometry, thin layer chromatography, Amplex Red phosphatidylcholine-specific phospholipase C, diacylglycerol",
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Tear analysis and lens-tear interactions : part II. Ocular lipids-nature and fate of meibomian gland phospholipids. / Campbell, Darren; Griffiths, Gareth; Tighe, Brian J.

In: Cornea, Vol. 30, No. 3, 03.2011, p. 325-332.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tear analysis and lens-tear interactions

T2 - part II. Ocular lipids-nature and fate of meibomian gland phospholipids

AU - Campbell, Darren

AU - Griffiths, Gareth

AU - Tighe, Brian J.

PY - 2011/3

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KW - diacylglycerol

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