Technical challenges of working with extracellular vesicles

Marcel I. Ramirez; Maria G. Amorim; Catarina Gadelha; Ivana Milic; Joshua A. Welsh; Vanessa M. Freitas; Muhammad Nawaz; Naveed Akbar; Yvonne Couch; Laura Makin; Fiona Cooke; Andre L. Vettore; Patricia X. Batista; Roberta Freezor; Julia A. Pezuk; Lívia Rosa-Fernandes; Ana Claudia O. Carreira; Andrew Devitt; Laura Jacobs; Israel T. Silva; Gillian Coakley; Diana N. Nunes; Dave Carter; Giuseppe Palmisano; Emmanuel Dias-Neto

doi:10.1039/c7nr08360b

Technical challenges of working with extracellular vesicles

Marcel I. Ramirez
, Maria G. Amorim
, Catarina Gadelha
, Ivana Milic
, Joshua A. Welsh
, Vanessa M. Freitas
, Muhammad Nawaz
, Naveed Akbar
, Yvonne Couch
, Laura Makin
, Fiona Cooke
, Andre L. Vettore
, Patricia X. Batista
, Roberta Freezor
, Julia A. Pezuk
, Lívia Rosa-Fernandes
, Ana Claudia O. Carreira
, Andrew Devitt
, Laura Jacobs
, Israel T. Silva

Gillian Coakley, Diana N. Nunes, Dave Carter, Giuseppe Palmisano, Emmanuel Dias-Neto^*

^*Corresponding author for this work

Fundação Instituto Oswaldo Cruz
Universidade Federal do Paraná
CIPE
Nottingham Trent University
University Hospital Southampton
Universidade de São Paulo
Göteborgs Universitet
Oxford Brookes University
Sir William Dunn School of Pathology
University of St. Andrews
Universidade Federal de São Paulo
London Metropolitan University
Universidade Anhanguera de São Paulo
University of Southern Denmark
Technische Universität München
University of Glasgow
Fondazione Santa Lucia

Research output: Contribution to journal › Review article › peer-review

483 Citations (SciVal)

83 Downloads (Pure)

Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Original language	English
Pages (from-to)	881-906
Number of pages	26
Journal	Nanoscale
Volume	10
Issue number	3
Early online date	13 Dec 2017
DOIs	https://doi.org/10.1039/c7nr08360b
Publication status	Published - 13 Dec 2017

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This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

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10.1039/c7nr08360bLicence: CC BY 3.0

Technical challenges of working with extracellular vesicles
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 1.23 MBLicence: CC BY 3.0

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Ramirez, M. I., Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., Cooke, F., Vettore, A. L., Batista, P. X., Freezor, R., Pezuk, J. A., Rosa-Fernandes, L., Carreira, A. C. O., Devitt, A., Jacobs, L., ... Dias-Neto, E. (2017). Technical challenges of working with extracellular vesicles. Nanoscale, 10(3), 881-906. https://doi.org/10.1039/c7nr08360b

@article{8dd60d2fdad040cdb9ba993e65c8490c,

title = "Technical challenges of working with extracellular vesicles",

abstract = "Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.",

author = "Ramirez, \{Marcel I.\} and Amorim, \{Maria G.\} and Catarina Gadelha and Ivana Milic and Welsh, \{Joshua A.\} and Freitas, \{Vanessa M.\} and Muhammad Nawaz and Naveed Akbar and Yvonne Couch and Laura Makin and Fiona Cooke and Vettore, \{Andre L.\} and Batista, \{Patricia X.\} and Roberta Freezor and Pezuk, \{Julia A.\} and L{\'i}via Rosa-Fernandes and Carreira, \{Ana Claudia O.\} and Andrew Devitt and Laura Jacobs and Silva, \{Israel T.\} and Gillian Coakley and Nunes, \{Diana N.\} and Dave Carter and Giuseppe Palmisano and Emmanuel Dias-Neto",

note = "This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. ",

year = "2017",

month = dec,

day = "13",

doi = "10.1039/c7nr08360b",

language = "English",

volume = "10",

pages = "881--906",

journal = "Nanoscale",

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Ramirez, MI, Amorim, MG, Gadelha, C, Milic, I, Welsh, JA, Freitas, VM, Nawaz, M, Akbar, N, Couch, Y, Makin, L, Cooke, F, Vettore, AL, Batista, PX, Freezor, R, Pezuk, JA, Rosa-Fernandes, L, Carreira, ACO, Devitt, A, Jacobs, L, Silva, IT, Coakley, G, Nunes, DN, Carter, D, Palmisano, G & Dias-Neto, E 2017, 'Technical challenges of working with extracellular vesicles', Nanoscale, vol. 10, no. 3, pp. 881-906. https://doi.org/10.1039/c7nr08360b

TY - JOUR

T1 - Technical challenges of working with extracellular vesicles

AU - Ramirez, Marcel I.

AU - Amorim, Maria G.

AU - Gadelha, Catarina

AU - Milic, Ivana

AU - Welsh, Joshua A.

AU - Freitas, Vanessa M.

AU - Nawaz, Muhammad

AU - Akbar, Naveed

AU - Couch, Yvonne

AU - Makin, Laura

AU - Cooke, Fiona

AU - Vettore, Andre L.

AU - Batista, Patricia X.

AU - Freezor, Roberta

AU - Pezuk, Julia A.

AU - Rosa-Fernandes, Lívia

AU - Carreira, Ana Claudia O.

AU - Devitt, Andrew

AU - Jacobs, Laura

AU - Silva, Israel T.

AU - Coakley, Gillian

AU - Nunes, Diana N.

AU - Carter, Dave

AU - Palmisano, Giuseppe

AU - Dias-Neto, Emmanuel

N1 - This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

PY - 2017/12/13

Y1 - 2017/12/13

N2 - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

AB - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

UR - https://www.scopus.com/pages/publications/85040917403

UR - http://pubs.rsc.org/en/content/articlelanding/2018/nr/c7nr08360b#!divAbstract

U2 - 10.1039/c7nr08360b

DO - 10.1039/c7nr08360b

M3 - Review article

AN - SCOPUS:85040917403

SN - 2040-3364

VL - 10

SP - 881

EP - 906

JO - Nanoscale

JF - Nanoscale

IS - 3

ER -

Technical challenges of working with extracellular vesicles

Abstract

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