Terminal galactose residues on transferrin are increased in mid-life adults compared to young adults

Christopher R. Dunston, Khujesta Choudhury, Helen R. Griffiths

Research output: Contribution to journalArticle

Abstract

Humans undergo biological ageing at different rates. This associates with functional decline in a number of physiological systems and increasing incidence of age-related pathologies. The discovery of robust biomarkers of ageing could be used to identify early divergence from a path of healthy ageing towards age-related disease. In the present study, we undertook proteomic analysis of plasma from healthy young men (mean age = 21.4 ± 1.5 years) and healthy mid-life men (mean age = 57.0 ±1.6 years). We identified twelve spots including transferrin, complement C3b and transthyretin that differed in abundance between the age groups. Transferrin spots showed an acidic pI shift in older males. Sandwich ELISAs were used to investigate the changes further. C3b levels were below the level of detection by ELISA and plasma concentrations of total transferrin or transthyretin were not different between the age groups studied here. However, analysis of transferrin N-glycan structures showed an increase in terminal galactose residues in older men, suggesting that the loss of terminal N-acetyl neuraminic acid residues contributes to the more acid pI of transferrin spots observed with age. Terminal galactosylation of transferrin may be a biomarker of healthy ageing and is now under investigation in the MARKAGE study.
LanguageEnglish
Pages3147-3153
Number of pages7
JournalProteomics
Volume12
Issue number21
Early online date12 Oct 2012
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Transferrin
Galactose
Young Adult
Aging of materials
Prealbumin
Biomarkers
Age Groups
Enzyme-Linked Immunosorbent Assay
Neuraminic Acids
Complement C3b
Plasmas
Pathology
Proteomics
Polysaccharides
Acids
Incidence

Keywords

  • n-acetyl neuraminic acid
  • sialic acid
  • n-glycosylation
  • biomarker
  • glycoproteomics

Cite this

Dunston, Christopher R. ; Choudhury, Khujesta ; Griffiths, Helen R. / Terminal galactose residues on transferrin are increased in mid-life adults compared to young adults. In: Proteomics. 2012 ; Vol. 12, No. 21. pp. 3147-3153.
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Terminal galactose residues on transferrin are increased in mid-life adults compared to young adults. / Dunston, Christopher R.; Choudhury, Khujesta; Griffiths, Helen R.

In: Proteomics, Vol. 12, No. 21, 11.2012, p. 3147-3153.

Research output: Contribution to journalArticle

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