Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Thannaphat Jenvoraphot; Boontharika Thapsukhon; Donraporn Daranarong; Robert Molloy; Chayarop Supanchart; Suttichai Krisanaprakornkit; Paul D. Topham; Brian Tighe; Anisa Mahomed; Winita Punyodom

doi:10.1021/acsapm.1c01794

Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Thannaphat Jenvoraphot, Boontharika Thapsukhon, Donraporn Daranarong, Robert Molloy, Chayarop Supanchart, Suttichai Krisanaprakornkit, Paul D. Topham, Brian Tighe, Anisa Mahomed, Winita Punyodom

Research output: Contribution to journal › Article › peer-review

Abstract

In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(l-lactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an anti-inflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31–56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.

Original language	English
Pages (from-to)	2459-2469
Number of pages	11
Journal	ACS Applied Polymer Materials
Volume	4
Issue number	4
Early online date	28 Mar 2022
DOIs	https://doi.org/10.1021/acsapm.1c01794
Publication status	Published - 8 Apr 2022

Bibliographical note

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright © 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01794

This research was financially supported by the Program Management Unit for Human Resources & Institutional Development, Research and Innovation, Office of National Higher Education Science Research and Innovation Policy
Council (NXPO) [grant number B16F640001], the Center for Innovation in Chemistry (PERCH-CIC) and Center of Excellence in Materials Science and Technology, Chiang Mai University. This project also received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL)

Keywords

antimicrobial activity
biodegradable
controlled release
electrospinning
periodontitis treatment
poly(l-lactide-co-ϵ-caprolactone)
tetracycline

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10.1021/acsapm.1c01794

Periodontal Manuscript_ACS Applied Polymer Materials_Final Rev
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright © 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01794
Accepted author manuscript, 1.64 MBLicence: CC BY-NC 4.0

Cite this

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title = "Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment",

abstract = "In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(l-lactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an anti-inflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31–56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.",

keywords = "antimicrobial activity, biodegradable, controlled release, electrospinning, periodontitis treatment, poly(l-lactide-co-ϵ-caprolactone), tetracycline",

author = "Thannaphat Jenvoraphot and Boontharika Thapsukhon and Donraporn Daranarong and Robert Molloy and Chayarop Supanchart and Suttichai Krisanaprakornkit and Topham, {Paul D.} and Brian Tighe and Anisa Mahomed and Winita Punyodom",

note = "This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright {\textcopyright} 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01794 This research was financially supported by the Program Management Unit for Human Resources & Institutional Development, Research and Innovation, Office of National Higher Education Science Research and Innovation Policy Council (NXPO) [grant number B16F640001], the Center for Innovation in Chemistry (PERCH-CIC) and Center of Excellence in Materials Science and Technology, Chiang Mai University. This project also received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL)",

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T1 - Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

AU - Jenvoraphot, Thannaphat

AU - Thapsukhon, Boontharika

AU - Daranarong, Donraporn

AU - Molloy, Robert

AU - Supanchart, Chayarop

AU - Krisanaprakornkit, Suttichai

AU - Topham, Paul D.

AU - Tighe, Brian

AU - Mahomed, Anisa

AU - Punyodom, Winita

N1 - This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright © 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01794 This research was financially supported by the Program Management Unit for Human Resources & Institutional Development, Research and Innovation, Office of National Higher Education Science Research and Innovation Policy Council (NXPO) [grant number B16F640001], the Center for Innovation in Chemistry (PERCH-CIC) and Center of Excellence in Materials Science and Technology, Chiang Mai University. This project also received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL)

PY - 2022/4/8

Y1 - 2022/4/8

N2 - In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(l-lactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an anti-inflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31–56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.

AB - In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(l-lactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an anti-inflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31–56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.

KW - antimicrobial activity

KW - biodegradable

KW - controlled release

KW - electrospinning

KW - periodontitis treatment

KW - poly(l-lactide-co-ϵ-caprolactone)

KW - tetracycline

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JO - ACS Applied Polymer Materials

JF - ACS Applied Polymer Materials

IS - 4

ER -

Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Abstract

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Keywords

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