Tetracycline-Loaded Electrospun Poly(L-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Thannaphat Jenvoraphot, Boontharika Thapsukhon, Donraporn Daranarong, Robert Molloy, Chayarop Supanchart, Suttichai Krisanaprakornkit, Paul D. Topham, Brian Tighe, Anisa Mahomed, Winita Punyodom

Research output: Contribution to journalArticlepeer-review


In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(l-lactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an anti-inflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31–56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.
Original languageEnglish
Pages (from-to)2459-2469
Number of pages11
JournalACS Applied Polymer Materials
Issue number4
Early online date28 Mar 2022
Publication statusPublished - 8 Apr 2022

Bibliographical note

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright © 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01794

This research was financially supported by the Program Management Unit for Human Resources & Institutional Development, Research and Innovation, Office of National Higher Education Science Research and Innovation Policy
Council (NXPO) [grant number B16F640001], the Center for Innovation in Chemistry (PERCH-CIC) and Center of Excellence in Materials Science and Technology, Chiang Mai University. This project also received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL)


  • antimicrobial activity
  • biodegradable
  • controlled release
  • electrospinning
  • periodontitis treatment
  • poly(l-lactide-co-ϵ-caprolactone)
  • tetracycline


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