The α5 gene product assembles with multiple acetylcholine receptor subunits to form distinctive receptor subtypes in brain

WilliamG Conroy, Ann B. Vernallis, Darwin K. Berg

Research output: Contribution to journalArticle

Abstract

The acetylcholine receptor (AChR) alpha 5 gene has been classified as a member of the AChR gene family based on sequence homology. Expression studies, however, have yet to identify a function for the alpha 5 gene product or even to demonstrate an interaction with known AChR subunits. We report here that the alpha 5 gene product is identical to the 49 kd protein previously found on immunoblots of AChRs purified from brain and ciliary ganglia. In brain the alpha 5 gene product is present both in alpha 3- and in alpha 4-based receptor subtypes, while in the ganglion it is found in an alpha 3-based receptor subtype concentrated in postsynaptic membrane. Immunoprecipitation experiments with subunit-specific monoclonal antibodies indicate that some native AChRs are likely to have at least three kinds of subunits, with two being of the alpha type. These findings support new views about the construction of AChRs in neurons.
Original languageEnglish
Pages (from-to)679-6691
Number of pages13
JournalNeuron
Volume9
Issue number4
DOIs
Publication statusPublished - Oct 1992

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Brain
  • Chick Embryo
  • Electric Organ
  • Electrophoresis, Polyacrylamide Gel
  • Electrophorus
  • Ganglia, Parasympathetic
  • Immunoblotting
  • Macromolecular Substances
  • Multigene Family
  • Muscles
  • Protein Biosynthesis
  • Receptors, Cholinergic
  • Recombinant Fusion Proteins

Fingerprint Dive into the research topics of 'The α5 gene product assembles with multiple acetylcholine receptor subunits to form distinctive receptor subtypes in brain'. Together they form a unique fingerprint.

Cite this