The development of attenuation compensation models of fluorescence spectroscopy signals

Victor V. Dremin, Evgeny A. Zherebtsov, Ilya E. Rafailov, Andrey Y. Vinokurov, Irina N. Novikova, Angelina I. Zherebtsova, Karina S. Litvinova, Andrey V. Dunaev

Research output: Chapter in Book/Report/Conference proceedingConference publication

Abstract

This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

Original languageEnglish
Title of host publicationThird International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
EditorsElina A. Genina, Valery V. Tuchin, Vladimir L. Derbov, et al
Place of PublicationBellingham, WA (US)
PublisherSPIE
Number of pages6
ISBN (Print)978-1-5106-0226-7
DOIs
Publication statusPublished - 21 Apr 2016
Event3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015 - Saratov, Russian Federation
Duration: 22 Sep 201525 Sep 2015

Publication series

NameSPIE Proceedings
PublisherSPIE
Volume9917
ISSN (Print)0277-786X
ISSN (Electronic)2410-9045

Conference

Conference3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015
CountryRussian Federation
CitySaratov
Period22/09/1525/09/15

Bibliographical note

-Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016).

Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

DOI: http://dx.doi.org/10.1117/12.2229451

Keywords

  • 3D model
  • correction spectra
  • diffuse reflectance spectroscopy
  • fluorescence spectroscopy
  • laser Doppler flowmetry
  • Monte Carlo simulation

Fingerprint Dive into the research topics of 'The development of attenuation compensation models of fluorescence spectroscopy signals'. Together they form a unique fingerprint.

  • Cite this

    Dremin, V. V., Zherebtsov, E. A., Rafailov, I. E., Vinokurov, A. Y., Novikova, I. N., Zherebtsova, A. I., Litvinova, K. S., & Dunaev, A. V. (2016). The development of attenuation compensation models of fluorescence spectroscopy signals. In E. A. Genina, V. V. Tuchin, V. L. Derbov, & et al (Eds.), Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS) [99170Y] (SPIE Proceedings; Vol. 9917). SPIE. https://doi.org/10.1117/12.2229451