The effect of antifoam addition on protein production yields

Sarah J. Routledge; Roslyn M. Bill

doi:10.1007/978-1-61779-770-5_9

The effect of antifoam addition on protein production yields

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.

Original language	English
Title of host publication	Recombinant protein production in yeast
Subtitle of host publication	methods and protocols
Editors	Roslyn M. Bill
Publisher	Humana Press
Pages	87-97
Number of pages	11
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5_9
Publication status	Published - 23 Apr 2012

Publication series

Name	Methods in molecular biology
Publisher	Humana Press
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Keywords

antifoam
protein
foam destruction yield
viability

Access to Document

10.1007/978-1-61779-770-5_9

Cite this

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The effect of antifoam addition on protein production yields. / Routledge, Sarah J.; Bill, Roslyn M.
Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 87-97 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

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T1 - The effect of antifoam addition on protein production yields

AU - Routledge, Sarah J.

AU - Bill, Roslyn M.

PY - 2012/4/23

Y1 - 2012/4/23

N2 - Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.

AB - Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.

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KW - viability

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BT - Recombinant protein production in yeast

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The effect of antifoam addition on protein production yields

Abstract

Publication series

Keywords

Access to Document

Other files and links

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Large-scale production of secreted proteins in Pichia pastoris

Optimising Pichia pastoris induction

Optimising yeast as a host for recombinant protein production (review)

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