The effect of antifoam addition on protein production yields

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.
Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages87-97
Number of pages11
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 23 Apr 2012

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Fingerprint

Antifoaming Agents
Recombinant Proteins
Batch Cell Culture Techniques
Pichia
Bioreactors
Proteins
Growth

Keywords

  • antifoam
  • protein
  • foam destruction yield
  • viability

Cite this

Routledge, S. J., & Bill, R. M. (2012). The effect of antifoam addition on protein production yields. In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 87-97). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_9
Routledge, Sarah J. ; Bill, Roslyn M. / The effect of antifoam addition on protein production yields. Recombinant protein production in yeast: methods and protocols. editor / Roslyn M. Bill. Humana Press, 2012. pp. 87-97 (Methods in molecular biology). (Springer protocols).
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Routledge, SJ & Bill, RM 2012, The effect of antifoam addition on protein production yields. in RM Bill (ed.), Recombinant protein production in yeast: methods and protocols. Methods in molecular biology, vol. 866, Springer protocols, Humana Press, pp. 87-97. https://doi.org/10.1007/978-1-61779-770-5_9

The effect of antifoam addition on protein production yields. / Routledge, Sarah J.; Bill, Roslyn M.

Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 87-97 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

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AU - Bill, Roslyn M.

PY - 2012/4/23

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N2 - Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.

AB - Pichia pastoris is a widely used host for recombinant protein production. The foaming associated with culturing it on a large scale is commonly prevented by the addition of chemical antifoaming agents or "antifoams." Unexpectedly, the addition of a range of antifoams to both shake flask and bioreactor cultures of P. pastoris has been shown to alter the total yield of the recombinant protein being produced. Possible explanations for this are that the presence of the antifoam increases the total amount of protein being produced and secreted per cell or that it increases the density of the culture. Antifoaming agents may therefore have specific effects on the growth and yield characteristics of recombinant cultures, in addition to their primary action as de-foamers.

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Routledge SJ, Bill RM. The effect of antifoam addition on protein production yields. In Bill RM, editor, Recombinant protein production in yeast: methods and protocols. Humana Press. 2012. p. 87-97. (Methods in molecular biology). (Springer protocols). https://doi.org/10.1007/978-1-61779-770-5_9