The effect of mechanical strain on protease production by keratinocytes

N Bhadal, I B Wall, S R Porter, S Broad, G E Lindahl, S Whawell, M P Lewis

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.

OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.

METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.

RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.

CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

Original languageEnglish
Pages (from-to)396-398
Number of pages3
JournalBritish Journal of Dermatology
Volume158
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Keratinocytes
Peptide Hydrolases
Collagen Type IV
Urokinase-Type Plasminogen Activator
Serum-Free Culture Media
Dermis
Collagen Type I
Wound Healing
Carcinogenesis
Skin
Membranes

Keywords

  • Cells, Cultured/metabolism
  • Humans
  • Keratinocytes/metabolism
  • Skin/metabolism
  • Stress, Mechanical
  • Urokinase-Type Plasminogen Activator/metabolism
  • Wound Healing/physiology

Cite this

Bhadal, N., Wall, I. B., Porter, S. R., Broad, S., Lindahl, G. E., Whawell, S., & Lewis, M. P. (2008). The effect of mechanical strain on protease production by keratinocytes. British Journal of Dermatology, 158(2), 396-398. https://doi.org/10.1111/j.1365-2133.2007.08341.x
Bhadal, N ; Wall, I B ; Porter, S R ; Broad, S ; Lindahl, G E ; Whawell, S ; Lewis, M P. / The effect of mechanical strain on protease production by keratinocytes. In: British Journal of Dermatology. 2008 ; Vol. 158, No. 2. pp. 396-398.
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Bhadal, N, Wall, IB, Porter, SR, Broad, S, Lindahl, GE, Whawell, S & Lewis, MP 2008, 'The effect of mechanical strain on protease production by keratinocytes', British Journal of Dermatology, vol. 158, no. 2, pp. 396-398. https://doi.org/10.1111/j.1365-2133.2007.08341.x

The effect of mechanical strain on protease production by keratinocytes. / Bhadal, N; Wall, I B; Porter, S R; Broad, S; Lindahl, G E; Whawell, S; Lewis, M P.

In: British Journal of Dermatology, Vol. 158, No. 2, 02.2008, p. 396-398.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of mechanical strain on protease production by keratinocytes

AU - Bhadal, N

AU - Wall, I B

AU - Porter, S R

AU - Broad, S

AU - Lindahl, G E

AU - Whawell, S

AU - Lewis, M P

PY - 2008/2

Y1 - 2008/2

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AB - BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

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KW - Humans

KW - Keratinocytes/metabolism

KW - Skin/metabolism

KW - Stress, Mechanical

KW - Urokinase-Type Plasminogen Activator/metabolism

KW - Wound Healing/physiology

U2 - 10.1111/j.1365-2133.2007.08341.x

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M3 - Article

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