The effect of mechanical strain on protease production by keratinocytes

N Bhadal; I B Wall; S R Porter; S Broad; G E Lindahl; S Whawell; M P Lewis

doi:10.1111/j.1365-2133.2007.08341.x

The effect of mechanical strain on protease production by keratinocytes

N Bhadal, I B Wall, S R Porter, S Broad, G E Lindahl, S Whawell, M P Lewis

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.

OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.

METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.

RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.

CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

Original language	English
Pages (from-to)	396-398
Number of pages	3
Journal	British Journal of Dermatology
Volume	158
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2133.2007.08341.x
Publication status	Published - Feb 2008

Keywords

Cells, Cultured/metabolism
Humans
Keratinocytes/metabolism
Skin/metabolism
Stress, Mechanical
Urokinase-Type Plasminogen Activator/metabolism
Wound Healing/physiology

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10.1111/j.1365-2133.2007.08341.x

Cite this

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title = "The effect of mechanical strain on protease production by keratinocytes",

abstract = "BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.",

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author = "N Bhadal and Wall, {I B} and Porter, {S R} and S Broad and Lindahl, {G E} and S Whawell and Lewis, {M P}",

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doi = "10.1111/j.1365-2133.2007.08341.x",

language = "English",

volume = "158",

pages = "396--398",

journal = "British Journal of Dermatology",

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T1 - The effect of mechanical strain on protease production by keratinocytes

AU - Bhadal, N

AU - Wall, I B

AU - Porter, S R

AU - Broad, S

AU - Lindahl, G E

AU - Whawell, S

AU - Lewis, M P

PY - 2008/2

Y1 - 2008/2

N2 - BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

AB - BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

KW - Cells, Cultured/metabolism

KW - Humans

KW - Keratinocytes/metabolism

KW - Skin/metabolism

KW - Stress, Mechanical

KW - Urokinase-Type Plasminogen Activator/metabolism

KW - Wound Healing/physiology

U2 - 10.1111/j.1365-2133.2007.08341.x

DO - 10.1111/j.1365-2133.2007.08341.x

M3 - Article

C2 - 18067479

SN - 0007-0963

VL - 158

SP - 396

EP - 398

JO - British Journal of Dermatology

JF - British Journal of Dermatology

IS - 2

ER -

The effect of mechanical strain on protease production by keratinocytes

Abstract

Keywords

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