The effect of mechanical strain on protease production by keratinocytes

N Bhadal, I B Wall, S R Porter, S Broad, G E Lindahl, S Whawell, M P Lewis

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated.

OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro.

METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied.

RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen.

CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

Original languageEnglish
Pages (from-to)396-398
Number of pages3
JournalBritish Journal of Dermatology
Volume158
Issue number2
DOIs
Publication statusPublished - Feb 2008

Keywords

  • Cells, Cultured/metabolism
  • Humans
  • Keratinocytes/metabolism
  • Skin/metabolism
  • Stress, Mechanical
  • Urokinase-Type Plasminogen Activator/metabolism
  • Wound Healing/physiology

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  • Cite this

    Bhadal, N., Wall, I. B., Porter, S. R., Broad, S., Lindahl, G. E., Whawell, S., & Lewis, M. P. (2008). The effect of mechanical strain on protease production by keratinocytes. British Journal of Dermatology, 158(2), 396-398. https://doi.org/10.1111/j.1365-2133.2007.08341.x