The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity

John Butler; Neil Smyth; Robert Broadbridge; Claire E. Council; Anthony G. Lee; Claire J. Stocker; David C. Hislop; Jonathan R.S. Arch; Michael A. Cawthorne; J. Malcolm East

doi:10.1016/j.abb.2015.01.027

The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity

John Butler, Neil Smyth, Robert Broadbridge, Claire E. Council, Anthony G. Lee, Claire J. Stocker, David C. Hislop, Jonathan R.S. Arch, Michael A. Cawthorne, J. Malcolm East^*

^*Corresponding author for this work

Aston Medical School

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

Original language	English
Pages (from-to)	26-31
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	569
DOIs	https://doi.org/10.1016/j.abb.2015.01.027
Publication status	Published - 1 Mar 2015

Bibliographical note

Publisher Copyright:
© 2015 The Authors.

Funding

We gratefully acknowledge the technical assistance of Bhavwanti Sheth and Caroline Smith, University of Southampton for the production of the transgenic mice; Ed Wargent, University of Buckingham, for the physiological analyses and the Wellcome Trust , UK (Grant # 086171/Z/08/Z ) for funding the study.

Funders	Funder number
University of Buckingham
Wellcome Trust	086171/Z/08/Z
University of Southampton

Keywords

Energy expenditure
High fat diet
Obesity
Sarcolipin
SERCA
Thermogenesis

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10.1016/j.abb.2015.01.027

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title = "The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity",

abstract = "Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.",

keywords = "Energy expenditure, High fat diet, Obesity, Sarcolipin, SERCA, Thermogenesis",

author = "John Butler and Neil Smyth and Robert Broadbridge and Council, {Claire E.} and Lee, {Anthony G.} and Stocker, {Claire J.} and Hislop, {David C.} and Arch, {Jonathan R.S.} and Cawthorne, {Michael A.} and {Malcolm East}, J.",

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doi = "10.1016/j.abb.2015.01.027",

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AU - Butler, John

AU - Smyth, Neil

AU - Broadbridge, Robert

AU - Council, Claire E.

AU - Lee, Anthony G.

AU - Stocker, Claire J.

AU - Hislop, David C.

AU - Arch, Jonathan R.S.

AU - Cawthorne, Michael A.

AU - Malcolm East, J.

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Y1 - 2015/3/1

N2 - Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

AB - Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

KW - Energy expenditure

KW - High fat diet

KW - Obesity

KW - Sarcolipin

KW - SERCA

KW - Thermogenesis

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The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity

Abstract

Bibliographical note

Funding

Keywords

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