The efficiency of encapsulation within surface rehydrated polymersomes

Anthony J. Parnell, N. Tzokova, Paul Topham, D.J. Adams, S. Adams, C. Fernyhough, A.J. Ryan, R.A.L. Jones

Research output: Contribution to journalArticle

Abstract

The key to the use of polymersomes as effective molecular delivery systems is in the ability to design processing routes that can efficiently encapsulate the molecular payload. We have evaluated various surface rehydration mechanisms for encapsulation, in each case characterizing the morphologies formed using DLS and confocal microscopy as well as determining the encapsulation efficiency for the hydrophilic dye Rhodamine B. In contrast to bulk methods, where the encapsulation efficiencies are low, we find that higher efficiencies can be obtained by the rehydration of thin films. We relate these results to the non-equilibrium mechanisms that underlie vesicle formation and discuss how an understanding of these mechanisms can help optimize encapsulation efficiencies. Our conclusion is that, even considering the good encapsulation efficiency, surface methods are still unsuitable for the massive scale-up needed when applied to commercial mass market molecular delivery scenarios. However, targeting more specialized applications for high value ingredients (like pharmaceuticals) might be more feasible.
Original languageEnglish
Pages (from-to)29-46
Number of pages18
JournalFaraday Discussions
Volume143
DOIs
Publication statusPublished - 28 Jul 2009

Fingerprint

Encapsulation
rhodamine B
delivery
Confocal microscopy
rhodamine
payloads
ingredients
Coloring Agents
dyes
routes
microscopy
Thin films
thin films
Processing
Pharmaceutical Preparations

Keywords

  • chemistry
  • polymersomes
  • surface rehydration mechanisms

Cite this

Parnell, A. J., Tzokova, N., Topham, P., Adams, D. J., Adams, S., Fernyhough, C., ... Jones, R. A. L. (2009). The efficiency of encapsulation within surface rehydrated polymersomes. Faraday Discussions, 143, 29-46. https://doi.org/10.1039/b902574j
Parnell, Anthony J. ; Tzokova, N. ; Topham, Paul ; Adams, D.J. ; Adams, S. ; Fernyhough, C. ; Ryan, A.J. ; Jones, R.A.L. / The efficiency of encapsulation within surface rehydrated polymersomes. In: Faraday Discussions. 2009 ; Vol. 143. pp. 29-46.
@article{e42ad86f5b8c4b2a876347c3b2fa03fc,
title = "The efficiency of encapsulation within surface rehydrated polymersomes",
abstract = "The key to the use of polymersomes as effective molecular delivery systems is in the ability to design processing routes that can efficiently encapsulate the molecular payload. We have evaluated various surface rehydration mechanisms for encapsulation, in each case characterizing the morphologies formed using DLS and confocal microscopy as well as determining the encapsulation efficiency for the hydrophilic dye Rhodamine B. In contrast to bulk methods, where the encapsulation efficiencies are low, we find that higher efficiencies can be obtained by the rehydration of thin films. We relate these results to the non-equilibrium mechanisms that underlie vesicle formation and discuss how an understanding of these mechanisms can help optimize encapsulation efficiencies. Our conclusion is that, even considering the good encapsulation efficiency, surface methods are still unsuitable for the massive scale-up needed when applied to commercial mass market molecular delivery scenarios. However, targeting more specialized applications for high value ingredients (like pharmaceuticals) might be more feasible.",
keywords = "chemistry, polymersomes, surface rehydration mechanisms",
author = "Parnell, {Anthony J.} and N. Tzokova and Paul Topham and D.J. Adams and S. Adams and C. Fernyhough and A.J. Ryan and R.A.L. Jones",
year = "2009",
month = "7",
day = "28",
doi = "10.1039/b902574j",
language = "English",
volume = "143",
pages = "29--46",
journal = "Faraday Discussions",
issn = "1359-6640",
publisher = "Royal Society of Chemistry",

}

Parnell, AJ, Tzokova, N, Topham, P, Adams, DJ, Adams, S, Fernyhough, C, Ryan, AJ & Jones, RAL 2009, 'The efficiency of encapsulation within surface rehydrated polymersomes', Faraday Discussions, vol. 143, pp. 29-46. https://doi.org/10.1039/b902574j

The efficiency of encapsulation within surface rehydrated polymersomes. / Parnell, Anthony J.; Tzokova, N.; Topham, Paul; Adams, D.J.; Adams, S.; Fernyhough, C.; Ryan, A.J.; Jones, R.A.L.

In: Faraday Discussions, Vol. 143, 28.07.2009, p. 29-46.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The efficiency of encapsulation within surface rehydrated polymersomes

AU - Parnell, Anthony J.

AU - Tzokova, N.

AU - Topham, Paul

AU - Adams, D.J.

AU - Adams, S.

AU - Fernyhough, C.

AU - Ryan, A.J.

AU - Jones, R.A.L.

PY - 2009/7/28

Y1 - 2009/7/28

N2 - The key to the use of polymersomes as effective molecular delivery systems is in the ability to design processing routes that can efficiently encapsulate the molecular payload. We have evaluated various surface rehydration mechanisms for encapsulation, in each case characterizing the morphologies formed using DLS and confocal microscopy as well as determining the encapsulation efficiency for the hydrophilic dye Rhodamine B. In contrast to bulk methods, where the encapsulation efficiencies are low, we find that higher efficiencies can be obtained by the rehydration of thin films. We relate these results to the non-equilibrium mechanisms that underlie vesicle formation and discuss how an understanding of these mechanisms can help optimize encapsulation efficiencies. Our conclusion is that, even considering the good encapsulation efficiency, surface methods are still unsuitable for the massive scale-up needed when applied to commercial mass market molecular delivery scenarios. However, targeting more specialized applications for high value ingredients (like pharmaceuticals) might be more feasible.

AB - The key to the use of polymersomes as effective molecular delivery systems is in the ability to design processing routes that can efficiently encapsulate the molecular payload. We have evaluated various surface rehydration mechanisms for encapsulation, in each case characterizing the morphologies formed using DLS and confocal microscopy as well as determining the encapsulation efficiency for the hydrophilic dye Rhodamine B. In contrast to bulk methods, where the encapsulation efficiencies are low, we find that higher efficiencies can be obtained by the rehydration of thin films. We relate these results to the non-equilibrium mechanisms that underlie vesicle formation and discuss how an understanding of these mechanisms can help optimize encapsulation efficiencies. Our conclusion is that, even considering the good encapsulation efficiency, surface methods are still unsuitable for the massive scale-up needed when applied to commercial mass market molecular delivery scenarios. However, targeting more specialized applications for high value ingredients (like pharmaceuticals) might be more feasible.

KW - chemistry

KW - polymersomes

KW - surface rehydration mechanisms

UR - http://www.scopus.com/inward/record.url?scp=70349296922&partnerID=8YFLogxK

UR - http://www.rsc.org/publishing/journals/FD/article.asp?doi=b902574j

U2 - 10.1039/b902574j

DO - 10.1039/b902574j

M3 - Article

VL - 143

SP - 29

EP - 46

JO - Faraday Discussions

JF - Faraday Discussions

SN - 1359-6640

ER -

Parnell AJ, Tzokova N, Topham P, Adams DJ, Adams S, Fernyhough C et al. The efficiency of encapsulation within surface rehydrated polymersomes. Faraday Discussions. 2009 Jul 28;143:29-46. https://doi.org/10.1039/b902574j