The elevation in circulating anti-angiogenic factors is independent of markers of neutrophil activation in preeclampsia

Wenda Ramma, Irina A. Buhimschi, Guomao Zhao, Antonette T. Dulay, Unzila Ali Nayeri, Catalin S. Buhimschi, Asif Ahmed

Research output: Contribution to journalArticlepeer-review

Abstract

Background - Severe preeclampsia is associated with increased neutrophil activation and elevated serum soluble endoglin (sEng) and soluble Flt-1 (sFlt-1) in the maternal circulation. To dissect the contribution of systemic inflammation and anti-angiogenic factors in preeclampsia, we investigated the relationships between the circulating markers of neutrophil activation and anti-angiogenic factors in severe preeclampsia or systemic inflammatory state during pregnancy.
Methods and results - Serum sEng, sFlt-1, placenta growth factor, interleukin-6 (IL-6), calprotectin, and plasma a-defensins concentrations were measured by ELISA in 88 women of similar gestational age stratified as: severe preeclampsia (sPE, n = 45), maternal systemic inflammatory response (SIR, n = 16) secondary to chorioamnionitis, pyelonephritis or appendicitis; and normotensive controls (CRL, n = 27). Neutrophil activation occurred in sPE and SIR, as a-defensins and calprotectin concentrations were two-fold higher in both groups compared to CRL (P < 0.05 for each). IL-6 concentrations were highest in SIR (P < 0.001), but were higher in sPE than in CRL (P < 0.01). sFlt-1 (P < 0.001) and sEng (P < 0.001) were ˜20-fold higher in sPE compared to CRL, but were not elevated in SIR. In women with sPE, anti-angiogenic factors were not correlated with markers of neutrophil activation (a-defensins, calprotectin) or inflammation (IL-6).
Conclusions - Increased systemic inflammation in sPE and SIR does not correlate with increased anti-angiogenic factors, which were specifically elevated in sPE indicating that excessive systemic inflammation is unlikely to be the main contributor to severe preeclampsia.
Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalAngiogenesis
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 2012

Bibliographical note

The original publication is available at www.springerlink.com
Creative Commons 2.0 Generic

Fingerprint

Dive into the research topics of 'The elevation in circulating anti-angiogenic factors is independent of markers of neutrophil activation in preeclampsia'. Together they form a unique fingerprint.

Cite this