The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation

Anna Khokhlova, Igor Zolotovskii, Dmitry Stoliarov, Svetlana Vorsina, Daria Liamina, Evgenia Sergeevna Pogodina, Andrei Fotiadi, Sergei G. Sokolovski, Yury Vladimirovich Saenko, Edik U. Rafailov

Research output: Contribution to journalArticle

Abstract

The mechanisms underlining the cell adaptive and/or activating oxidative stress, called low level light or photobiomodulation therapies (PBMT), still remain unclear for the near-infrared spectrum range (750-3000 nm), especially for the 1265-1270 nm range (highest absorption by molecular oxygen). It is most probable that the mitochondria may also appear to be the main target for these wavelengths. It is known that mitochondria can generate ROS under visible and 800-1060 nm spectrum range irradiation, which in turn control voltage-dependent anion channels (VDAC). Here we investigated cellular damage regarding VDAC activity, level of oxidative stress, malondialdehyde content, cell viability, mitochondrial potential and mass, GSH level, mitochondrial and nuclear DNA damage in the cancer cell culture exposed to low-level laser irradiation at 1265 nm. We used a continuous wave laser with output power 4 mW; the energy densities employed were 0.3-9.45 J/cm 2. We observed that the laser radiation at 1265 nm can induce the oxidative stress, enhance apoptosis, and disturb mitochondrial functioning at the energy density of 9.54 J/cm 2. In addition, inhibition of VDAC enhances the observed effects. It has been shown that the laser irradiation at 1265 nm damages mitochondrial DNA but does not affect the nuclear DNA. The performed experiments bring us to the conclusion that the laser irradiation at 1265 nm can affect cells through mitochondrial damage and the inhibition of VDAC enhances effects of PBMT.

Original languageEnglish
Article number8410463
JournalIEEE Journal of Selected Topics in Quantum Electronics
Volume25
Issue number1
Early online date12 Jul 2018
DOIs
Publication statusPublished - 1 Jan 2019

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Laser beam effects
Cell culture
infrared lasers
Dosimetry
Oxidative stress
Negative ions
cancer
anions
damage
dosage
Mitochondria
irradiation
DNA
mitochondria
deoxyribonucleic acid
electric potential
therapy
Electric potential
flux density
lasers

Bibliographical note

© 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

Keywords

  • Biophotonics
  • cancer cell
  • near-infrared lasers
  • photobiomodulation therapy

Cite this

Khokhlova, Anna ; Zolotovskii, Igor ; Stoliarov, Dmitry ; Vorsina, Svetlana ; Liamina, Daria ; Pogodina, Evgenia Sergeevna ; Fotiadi, Andrei ; Sokolovski, Sergei G. ; Saenko, Yury Vladimirovich ; Rafailov, Edik U. / The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation. In: IEEE Journal of Selected Topics in Quantum Electronics. 2019 ; Vol. 25, No. 1.
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The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation. / Khokhlova, Anna; Zolotovskii, Igor; Stoliarov, Dmitry; Vorsina, Svetlana; Liamina, Daria; Pogodina, Evgenia Sergeevna; Fotiadi, Andrei; Sokolovski, Sergei G.; Saenko, Yury Vladimirovich; Rafailov, Edik U.

In: IEEE Journal of Selected Topics in Quantum Electronics, Vol. 25, No. 1, 8410463, 01.01.2019.

Research output: Contribution to journalArticle

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T1 - The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation

AU - Khokhlova, Anna

AU - Zolotovskii, Igor

AU - Stoliarov, Dmitry

AU - Vorsina, Svetlana

AU - Liamina, Daria

AU - Pogodina, Evgenia Sergeevna

AU - Fotiadi, Andrei

AU - Sokolovski, Sergei G.

AU - Saenko, Yury Vladimirovich

AU - Rafailov, Edik U.

N1 - © 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The mechanisms underlining the cell adaptive and/or activating oxidative stress, called low level light or photobiomodulation therapies (PBMT), still remain unclear for the near-infrared spectrum range (750-3000 nm), especially for the 1265-1270 nm range (highest absorption by molecular oxygen). It is most probable that the mitochondria may also appear to be the main target for these wavelengths. It is known that mitochondria can generate ROS under visible and 800-1060 nm spectrum range irradiation, which in turn control voltage-dependent anion channels (VDAC). Here we investigated cellular damage regarding VDAC activity, level of oxidative stress, malondialdehyde content, cell viability, mitochondrial potential and mass, GSH level, mitochondrial and nuclear DNA damage in the cancer cell culture exposed to low-level laser irradiation at 1265 nm. We used a continuous wave laser with output power 4 mW; the energy densities employed were 0.3-9.45 J/cm 2. We observed that the laser radiation at 1265 nm can induce the oxidative stress, enhance apoptosis, and disturb mitochondrial functioning at the energy density of 9.54 J/cm 2. In addition, inhibition of VDAC enhances the observed effects. It has been shown that the laser irradiation at 1265 nm damages mitochondrial DNA but does not affect the nuclear DNA. The performed experiments bring us to the conclusion that the laser irradiation at 1265 nm can affect cells through mitochondrial damage and the inhibition of VDAC enhances effects of PBMT.

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