The phytochrome control of the cAMP endogenous level in oat seedlings

O. V. Molchan*, S. G. Sokolovsky, I. D. Volotovsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The activity of adenylate cyclase (AC, EC was studied in oat (Avena sativa L.) seedlings. The following conditions were optimal for AC activity: a substrate (Mg2+-ATP) concentration of 0.75 mM; a temperature of 20-25°C, and pH 7.5. AC activity was detected in all subcellular fractions. The highest activity was observed in the microsomal fraction. GTP, its nonhydrolyzable analogs, and forskolin substantially enhanced the AC activity, whereas extracellular Ca2+ had no effect. The AC is suggested to exist in plant tissues in a membrane-bound form. The endogenous cAMP level was shown to depend on seedling illumination: it was far lower in etiolated seedlings and those adapted to darkness. Red and far-red light affected the endogenous cAMP content, indicating the possible involvement of cAMP in the phytochrome signaling pathway.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalRussian Journal of Plant Physiology
Issue number4
Publication statusPublished - Jul 2000

Bibliographical note

Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.


  • Adenylate cyclase activity
  • Avena sativa
  • cAMP
  • Phytochrome
  • Regulation
  • Seedlings


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