The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastoma cells after peroxide insult, which is associated with increased survival

Melissa M. Grant, Vicki S. Barber, Helen R. Griffiths*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Oxidative stress and free radical production have been implicated in Alzheimer's disease, where low levels of the antioxidant vitamin C (ascorbate) have been shown to be associated with the disease. In this study, neuroblastoma SH-SY5Y cells were treated with hydrogen peroxide in the presence of ascorbate in order to elucidate the me0chanism(s) of protection against oxidative stress afforded by ascorbate. Protein oxidation, glutathione levels, cell viability and the effects on the proteome and its oxidized counterpart were monitored. SH-SY5Y cells treated with ascorbate prior to co-incubation with peroxide showed increased viability in comparison to cells treated with peroxide alone. This dual treatment also caused an increase in protein carbonyl content and a decrease in glutathione levels within the cells. Proteins, extracted from SH-SY5Y cells that were treated with either ascorbate or peroxide alone or with ascorbate prior to peroxide, were separated by two-dimensional gel electrophoresis and analyzed for oxidation. Co-incubation for 24 hours decreased the number of oxidised proteins (e.g. acyl CoA oxidase 3) and induced brain derived neurotrophic factor (BDNF) expression. Enhanced expression of BDNF may contribute to the protective effects of ascorbate against oxidative stress in neuronal cells.

Original languageEnglish
Pages (from-to)534-540
Number of pages7
JournalProteomics
Volume5
Issue number2
DOIs
Publication statusPublished - 3 Jan 2005

Bibliographical note

This is the pre-peer reviewed version of the following article: Grant, Melissa M.; Barber, Vicki S. and Griffiths, Helen R. (2005) The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastorna cells after peroxide insult, which is associated with increased survival. Proteomics, 5 (2). pp. 534-540. ISSN 1862-8346, which has been published in final form at http://www3.interscience.wiley.com/journal/109861619/abstract

Keywords

  • ascorbate
  • hydrogen peroxide
  • neurotoxicity
  • SH-SY5Y

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