The role of the extracellular loops of the CGRP receptor, a family B GPCR

James Barwell, Michael J. Woolley, Mark Wheatley, Alex C. Conner, David R Poyner

Research output: Contribution to journalArticle

Abstract

The CGRP (calcitonin gene-related peptide) receptor is a family B GPCR (G-protein-coupled receptor). It consists of a GPCR, CLR (calcitonin receptor-like receptor) and an accessory protein, RAMP1 (receptor activity-modifying protein 1). RAMP1 is needed for CGRP binding and also cell-surface expression of CLR. There have been few systematic studies of the ECLs (extracellular loops) of family B GPCRs. However, they are likely to be especially important for the interaction of the N-termini of the peptide agonists that are the natural agonists for these receptors. We have carried out alanine scans on all three ECLs of CLR, as well as their associated juxtamembrane regions. Residues within all three loops influence CGRP binding and receptor activation. Mutation of Ala203 and Ala206 on ECL1 to leucine increased the affinity of CGRP. Residues at the top of TM (transmembrane) helices 2 and 3 influenced CGRP binding and receptor activation. L351A and E357A in TM6/ECL3 reduced receptor expression and may be needed for CLR association with RAMP1. ECL2 seems especially important for CLR function; of the 16 residues so far examined in this loop, eight residues reduce the potency of CGRP at stimulating cAMP production when mutated to alanine.
Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalBiochemical Society Transactions
Volume40
Issue number2
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide Receptors
Calcitonin Gene-Related Peptide
Receptor Activity-Modifying Protein 1
G-Protein-Coupled Receptors
Alanine
Chemical activation
Accessories
Leucine
Association reactions
Proteins
Peptides
Mutation

Keywords

  • alanine scan
  • calcitonin
  • mutagenesis
  • receptor activity-modifying protein
  • secretin-like G-protein-coupled receptor
  • structure–activity relationship

Cite this

Barwell, James ; Woolley, Michael J. ; Wheatley, Mark ; Conner, Alex C. ; Poyner, David R. / The role of the extracellular loops of the CGRP receptor, a family B GPCR. In: Biochemical Society Transactions. 2012 ; Vol. 40, No. 2. pp. 433-437.
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The role of the extracellular loops of the CGRP receptor, a family B GPCR. / Barwell, James; Woolley, Michael J.; Wheatley, Mark; Conner, Alex C.; Poyner, David R.

In: Biochemical Society Transactions, Vol. 40, No. 2, 04.2012, p. 433-437.

Research output: Contribution to journalArticle

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