TY - JOUR
T1 - The role of tissue transglutaminase in 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity in differentiated human SH-SY5Y neuroblastoma cells
AU - Beck, Katy E.
AU - De Girolamo, Luigi A.
AU - Griffin, Martin
AU - Billett, E. Ellen
PY - 2006/9/11
Y1 - 2006/9/11
N2 - Tissue transglutaminase (TG2) can induce post-translational modification of proteins, resulting in protein cross-linking or incorporation of polyamines into substrates, and can also function as a signal transducing G protein. The role of TG2 in the formation of insoluble cross-links has led to its implication in some neurodegenerative conditions. Exposure of pre-differentiated SH-SY5Y cells to the Parkinsonian neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+) resulted in significant dose-dependent reductions in TG2 protein levels, measured by probing Western blots with a TG2-specific antibody. Transglutaminase (TG) transamidating activity, on the other hand, monitored by incorporation of a polyamine pseudo-substrate into cellular proteins, was increased. Inhibitors of TG (putrescine) and TG2 (R283) exacerbated MPP+ toxicity, suggesting that activation of TG2 may promote a survival response in this toxicity paradigm.
AB - Tissue transglutaminase (TG2) can induce post-translational modification of proteins, resulting in protein cross-linking or incorporation of polyamines into substrates, and can also function as a signal transducing G protein. The role of TG2 in the formation of insoluble cross-links has led to its implication in some neurodegenerative conditions. Exposure of pre-differentiated SH-SY5Y cells to the Parkinsonian neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+) resulted in significant dose-dependent reductions in TG2 protein levels, measured by probing Western blots with a TG2-specific antibody. Transglutaminase (TG) transamidating activity, on the other hand, monitored by incorporation of a polyamine pseudo-substrate into cellular proteins, was increased. Inhibitors of TG (putrescine) and TG2 (R283) exacerbated MPP+ toxicity, suggesting that activation of TG2 may promote a survival response in this toxicity paradigm.
KW - Parkinson's disease
KW - MPP+
KW - tissue transglutaminase
KW - SH-SY5Y human neuroblastoma
KW - cell viability
UR - http://www.scopus.com/inward/record.url?scp=33746472503&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2006.06.061
DO - 10.1016/j.neulet.2006.06.061
M3 - Article
C2 - 16876317
SN - 0304-3940
VL - 405
SP - 46
EP - 51
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -