The significance of hand wash compliance on the transfer of dermal lipids in contact lens wear

Darren Campbell, A. Mann, O. Hunt, L.J.R. Santos

Research output: Contribution to journalArticle

Abstract

Aim: The aim of this study was to assess the impact of hand washing regimes on lipid transference to contact lenses. The presence of lipids on contact lenses can affect visual acuity and enhance spoilation. Additionally, they may even mediate and foster microbial transfer and serve as a marker of potential dermal contamination. Methods and materials: A social hand wash and the Royal College of Nursing (RCN) hand wash were investigated. A 'no-wash regime' was used as control. The transfer of lipids from the hand was assessed by Thin Layer Chromatography (TLC). Lipid transference to the contact lenses was studied through fluorescence spectroscopy (FS). Results: Iodine staining, for presence of lipids, on TLC plates indicated the 'no-wash regime' score averaged at 3.4 ± 0.8, the social wash averaged at 2.2 ± 0.9 and the RCN averaged at 1.2 ± 0.3 on a scale of 1-4. The FS of lipids on contact lenses for 'no washing' presented an average of 28.47 ± 10.54 fluorescence units (FU), the social wash presented an average of 13.52 ± 11.12. FU and the RCN wash presented a much lower average 6.47 ± 4.26. FU. Conclusions: This work demonstrates how the method used for washing the hands can affect the concentration of lipids, and the transfer of these lipids onto contact lenses. A regime of hand washing for contact lens users should be standardised to help reduce potentially transferable species present on the hands.
Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalContact Lens and Anterior Eye
Volume35
Issue number2
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Contact Lenses
Compliance
Hand
Lipids
Skin
Hand Disinfection
Fluorescence
Fluorescence Spectrometry
Thin Layer Chromatography
Nursing
Iodine
Visual Acuity
Staining and Labeling

Keywords

  • compliance
  • contamination
  • lens handling
  • lipids
  • dermal

Cite this

@article{219056908d0e4401b018d462ea02938a,
title = "The significance of hand wash compliance on the transfer of dermal lipids in contact lens wear",
abstract = "Aim: The aim of this study was to assess the impact of hand washing regimes on lipid transference to contact lenses. The presence of lipids on contact lenses can affect visual acuity and enhance spoilation. Additionally, they may even mediate and foster microbial transfer and serve as a marker of potential dermal contamination. Methods and materials: A social hand wash and the Royal College of Nursing (RCN) hand wash were investigated. A 'no-wash regime' was used as control. The transfer of lipids from the hand was assessed by Thin Layer Chromatography (TLC). Lipid transference to the contact lenses was studied through fluorescence spectroscopy (FS). Results: Iodine staining, for presence of lipids, on TLC plates indicated the 'no-wash regime' score averaged at 3.4 ± 0.8, the social wash averaged at 2.2 ± 0.9 and the RCN averaged at 1.2 ± 0.3 on a scale of 1-4. The FS of lipids on contact lenses for 'no washing' presented an average of 28.47 ± 10.54 fluorescence units (FU), the social wash presented an average of 13.52 ± 11.12. FU and the RCN wash presented a much lower average 6.47 ± 4.26. FU. Conclusions: This work demonstrates how the method used for washing the hands can affect the concentration of lipids, and the transfer of these lipids onto contact lenses. A regime of hand washing for contact lens users should be standardised to help reduce potentially transferable species present on the hands.",
keywords = "compliance, contamination, lens handling, lipids, dermal",
author = "Darren Campbell and A. Mann and O. Hunt and L.J.R. Santos",
note = "Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2012",
month = "4",
doi = "10.1016/j.clae.2011.11.004",
language = "English",
volume = "35",
pages = "71--76",
journal = "Contact Lens and Anterior Eye",
issn = "1367-0484",
publisher = "Elsevier",
number = "2",

}

The significance of hand wash compliance on the transfer of dermal lipids in contact lens wear. / Campbell, Darren; Mann, A.; Hunt, O.; Santos, L.J.R.

In: Contact Lens and Anterior Eye, Vol. 35, No. 2, 04.2012, p. 71-76.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The significance of hand wash compliance on the transfer of dermal lipids in contact lens wear

AU - Campbell, Darren

AU - Mann, A.

AU - Hunt, O.

AU - Santos, L.J.R.

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/4

Y1 - 2012/4

N2 - Aim: The aim of this study was to assess the impact of hand washing regimes on lipid transference to contact lenses. The presence of lipids on contact lenses can affect visual acuity and enhance spoilation. Additionally, they may even mediate and foster microbial transfer and serve as a marker of potential dermal contamination. Methods and materials: A social hand wash and the Royal College of Nursing (RCN) hand wash were investigated. A 'no-wash regime' was used as control. The transfer of lipids from the hand was assessed by Thin Layer Chromatography (TLC). Lipid transference to the contact lenses was studied through fluorescence spectroscopy (FS). Results: Iodine staining, for presence of lipids, on TLC plates indicated the 'no-wash regime' score averaged at 3.4 ± 0.8, the social wash averaged at 2.2 ± 0.9 and the RCN averaged at 1.2 ± 0.3 on a scale of 1-4. The FS of lipids on contact lenses for 'no washing' presented an average of 28.47 ± 10.54 fluorescence units (FU), the social wash presented an average of 13.52 ± 11.12. FU and the RCN wash presented a much lower average 6.47 ± 4.26. FU. Conclusions: This work demonstrates how the method used for washing the hands can affect the concentration of lipids, and the transfer of these lipids onto contact lenses. A regime of hand washing for contact lens users should be standardised to help reduce potentially transferable species present on the hands.

AB - Aim: The aim of this study was to assess the impact of hand washing regimes on lipid transference to contact lenses. The presence of lipids on contact lenses can affect visual acuity and enhance spoilation. Additionally, they may even mediate and foster microbial transfer and serve as a marker of potential dermal contamination. Methods and materials: A social hand wash and the Royal College of Nursing (RCN) hand wash were investigated. A 'no-wash regime' was used as control. The transfer of lipids from the hand was assessed by Thin Layer Chromatography (TLC). Lipid transference to the contact lenses was studied through fluorescence spectroscopy (FS). Results: Iodine staining, for presence of lipids, on TLC plates indicated the 'no-wash regime' score averaged at 3.4 ± 0.8, the social wash averaged at 2.2 ± 0.9 and the RCN averaged at 1.2 ± 0.3 on a scale of 1-4. The FS of lipids on contact lenses for 'no washing' presented an average of 28.47 ± 10.54 fluorescence units (FU), the social wash presented an average of 13.52 ± 11.12. FU and the RCN wash presented a much lower average 6.47 ± 4.26. FU. Conclusions: This work demonstrates how the method used for washing the hands can affect the concentration of lipids, and the transfer of these lipids onto contact lenses. A regime of hand washing for contact lens users should be standardised to help reduce potentially transferable species present on the hands.

KW - compliance

KW - contamination

KW - lens handling

KW - lipids

KW - dermal

UR - http://www.scopus.com/inward/record.url?scp=84857796004&partnerID=8YFLogxK

U2 - 10.1016/j.clae.2011.11.004

DO - 10.1016/j.clae.2011.11.004

M3 - Article

AN - SCOPUS:84857796004

VL - 35

SP - 71

EP - 76

JO - Contact Lens and Anterior Eye

JF - Contact Lens and Anterior Eye

SN - 1367-0484

IS - 2

ER -