The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas

Jindong Chen; Weng-Onn Lui; Michele D. Vos; Geoffrey J. Clark; Masayuki Takahashi; Jacqueline Schoumans; Sok Kean Khoo; David Petillo; Todd Lavery; Jun Sugimura; Dewi Astuti; Chun Zhang; Susumu Kagawa; Eamonn R. Maher; Catharina Larsson; Arthur S. Alberts; Hiro-omi Kanayama; Bin Tean Teh

doi:10.1016/s1535-6108(03)00269-1

The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas

Jindong Chen, Weng-Onn Lui, Michele D. Vos, Geoffrey J. Clark, Masayuki Takahashi, Jacqueline Schoumans, Sok Kean Khoo, David Petillo, Todd Lavery, Jun Sugimura, Dewi Astuti, Chun Zhang, Susumu Kagawa, Eamonn R. Maher, Catharina Larsson, Arthur S. Alberts, Hiro-omi Kanayama, Bin Tean Teh

Aston Medical School

Research output: Contribution to journal › Article › peer-review

100 Citations (Scopus)

Abstract

By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.

Original language	English
Pages (from-to)	405-13
Number of pages	9
Journal	Cancer cell
Volume	4
Issue number	5
DOIs	https://doi.org/10.1016/s1535-6108(03)00269-1
Publication status	Published - Nov 2003

Keywords

Adaptor Proteins, Signal Transducing
Adenocarcinoma, Clear Cell/genetics
Animals
Apoptosis Regulatory Proteins
Base Sequence
Carcinoma, Renal Cell/genetics
Cell Adhesion Molecules, Neuronal/genetics
Cell Division/physiology
Cells, Cultured
Cloning, Molecular
DNA Methylation
GPI-Linked Proteins
Gene Expression Regulation, Neoplastic/physiology
Humans
Molecular Sequence Data
Monomeric GTP-Binding Proteins/genetics

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Chen, J., Lui, W.-O., Vos, M. D., Clark, G. J., Takahashi, M., Schoumans, J., Khoo, S. K., Petillo, D., Lavery, T., Sugimura, J., Astuti, D., Zhang, C., Kagawa, S., Maher, E. R., Larsson, C., Alberts, A. S., Kanayama, H., & Teh, B. T. (2003). The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas. Cancer cell, 4(5), 405-13. https://doi.org/10.1016/s1535-6108(03)00269-1

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title = "The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas",

abstract = "By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.",

keywords = "Adaptor Proteins, Signal Transducing, Adenocarcinoma, Clear Cell/genetics, Animals, Apoptosis Regulatory Proteins, Base Sequence, Carcinoma, Renal Cell/genetics, Cell Adhesion Molecules, Neuronal/genetics, Cell Division/physiology, Cells, Cultured, Cloning, Molecular, DNA Methylation, GPI-Linked Proteins, Gene Expression Regulation, Neoplastic/physiology, Humans, Molecular Sequence Data, Monomeric GTP-Binding Proteins/genetics",

author = "Jindong Chen and Weng-Onn Lui and Vos, {Michele D.} and Clark, {Geoffrey J.} and Masayuki Takahashi and Jacqueline Schoumans and Khoo, {Sok Kean} and David Petillo and Todd Lavery and Jun Sugimura and Dewi Astuti and Chun Zhang and Susumu Kagawa and Maher, {Eamonn R.} and Catharina Larsson and Alberts, {Arthur S.} and Hiro-omi Kanayama and Teh, {Bin Tean}",

year = "2003",

month = nov,

doi = "10.1016/s1535-6108(03)00269-1",

language = "English",

volume = "4",

pages = "405--13",

journal = "Cancer cell",

issn = "1535-6108",

publisher = "Cell Press",

number = "5",

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Chen, J, Lui, W-O, Vos, MD, Clark, GJ, Takahashi, M, Schoumans, J, Khoo, SK, Petillo, D, Lavery, T, Sugimura, J, Astuti, D, Zhang, C, Kagawa, S, Maher, ER, Larsson, C, Alberts, AS, Kanayama, H & Teh, BT 2003, 'The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas', Cancer cell, vol. 4, no. 5, pp. 405-13. https://doi.org/10.1016/s1535-6108(03)00269-1

TY - JOUR

T1 - The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas

AU - Chen, Jindong

AU - Lui, Weng-Onn

AU - Vos, Michele D.

AU - Clark, Geoffrey J.

AU - Takahashi, Masayuki

AU - Schoumans, Jacqueline

AU - Khoo, Sok Kean

AU - Petillo, David

AU - Lavery, Todd

AU - Sugimura, Jun

AU - Astuti, Dewi

AU - Zhang, Chun

AU - Kagawa, Susumu

AU - Maher, Eamonn R.

AU - Larsson, Catharina

AU - Alberts, Arthur S.

AU - Kanayama, Hiro-omi

AU - Teh, Bin Tean

PY - 2003/11

Y1 - 2003/11

N2 - By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.

AB - By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.

KW - Adaptor Proteins, Signal Transducing

KW - Adenocarcinoma, Clear Cell/genetics

KW - Animals

KW - Apoptosis Regulatory Proteins

KW - Base Sequence

KW - Carcinoma, Renal Cell/genetics

KW - Cell Adhesion Molecules, Neuronal/genetics

KW - Cell Division/physiology

KW - Cells, Cultured

KW - Cloning, Molecular

KW - DNA Methylation

KW - GPI-Linked Proteins

KW - Gene Expression Regulation, Neoplastic/physiology

KW - Humans

KW - Molecular Sequence Data

KW - Monomeric GTP-Binding Proteins/genetics

UR - https://linkinghub.elsevier.com/retrieve/pii/S1535610803002691

U2 - 10.1016/s1535-6108(03)00269-1

DO - 10.1016/s1535-6108(03)00269-1

M3 - Article

C2 - 14667507

SN - 1535-6108

VL - 4

SP - 405

EP - 413

JO - Cancer cell

JF - Cancer cell

IS - 5

ER -

The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas

Abstract

Keywords

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