The use of amino acids to enhance the aerosolisation of spray-dried powders for pulmonary gene therapy

Hao-Ying Li, Peter C. Seville*, I.J. Williamson, J.C. Birchall

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Background Pulmonary delivery of gene therapy offers the potential for the treatment of a range of lung conditions, including cystic fibrosis, asthma and lung cancer. Spray-drying may be used to prepare dry powders for inhalation; however, aerosolisation of such powders is limited, resulting in poor lung deposition and biological functionality. In this study, we examine the use of amino acids (arginine, aspartic acid, threonine, phenylalanine) to enhance the aerosolisation of spray-dried powders containing model non-viral gene vectors. Methods Lipid/polycation/pDNA (LPD) vectors, in the presence or absence of amino acids, were dispersed in lactose solutions, and spray-dried to produce appropriately sized dry powders. Scanning electron microscopy and laser diffraction were used to determine particle morphology and diameter, respectively. Gel electrophoresis was used to examine the influence of amino acids on the structural integrity of the LPD complex. In vitro cell (A.549) transfection was used to determine the biological functionality of the dry powders, and the in vitro aerosolisation performance was assessed using a multistage liquid impinger (MSLI). Results Both gel electrophoresis and in vitro cell transfection indicated that certain amino acids (aspartic acid, threonine) can adversely affect the integrity and biological functionality of the LPD complex. All amino acids significantly increased the aerosolisation of the powder, with the arginine and phenylalanine powders showing optimal deposition in the lower stages of the MSLI. Conclusions Amino acids can be used to enhance the aerosolisation of spray-dried powders for respiratory gene delivery, allowing the development of stable and viable formulations for pulmonary gene therapy.
Original languageEnglish
Pages (from-to)343-353
Number of pages11
JournalJournal of Gene Medicine
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Genetic Therapy
Powders
Amino Acids
Lung
Threonine
Phenylalanine
Lipids
Aspartic Acid
Transfection
Arginine
Electrophoresis
Gels
Lactose
Transmission Electron Microscopy
Cystic Fibrosis
Electron Scanning Microscopy
Inhalation
Genes
Lung Neoplasms
Lasers

Keywords

  • LPD complex
  • spray-drying
  • pulmonary deposition
  • amino acids
  • gene delivery
  • non-viral

Cite this

Li, Hao-Ying ; Seville, Peter C. ; Williamson, I.J. ; Birchall, J.C. / The use of amino acids to enhance the aerosolisation of spray-dried powders for pulmonary gene therapy. In: Journal of Gene Medicine. 2005 ; Vol. 7, No. 3. pp. 343-353.
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The use of amino acids to enhance the aerosolisation of spray-dried powders for pulmonary gene therapy. / Li, Hao-Ying; Seville, Peter C.; Williamson, I.J.; Birchall, J.C.

In: Journal of Gene Medicine, Vol. 7, No. 3, 03.2005, p. 343-353.

Research output: Contribution to journalArticle

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N2 - Background Pulmonary delivery of gene therapy offers the potential for the treatment of a range of lung conditions, including cystic fibrosis, asthma and lung cancer. Spray-drying may be used to prepare dry powders for inhalation; however, aerosolisation of such powders is limited, resulting in poor lung deposition and biological functionality. In this study, we examine the use of amino acids (arginine, aspartic acid, threonine, phenylalanine) to enhance the aerosolisation of spray-dried powders containing model non-viral gene vectors. Methods Lipid/polycation/pDNA (LPD) vectors, in the presence or absence of amino acids, were dispersed in lactose solutions, and spray-dried to produce appropriately sized dry powders. Scanning electron microscopy and laser diffraction were used to determine particle morphology and diameter, respectively. Gel electrophoresis was used to examine the influence of amino acids on the structural integrity of the LPD complex. In vitro cell (A.549) transfection was used to determine the biological functionality of the dry powders, and the in vitro aerosolisation performance was assessed using a multistage liquid impinger (MSLI). Results Both gel electrophoresis and in vitro cell transfection indicated that certain amino acids (aspartic acid, threonine) can adversely affect the integrity and biological functionality of the LPD complex. All amino acids significantly increased the aerosolisation of the powder, with the arginine and phenylalanine powders showing optimal deposition in the lower stages of the MSLI. Conclusions Amino acids can be used to enhance the aerosolisation of spray-dried powders for respiratory gene delivery, allowing the development of stable and viable formulations for pulmonary gene therapy.

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