The use of erythrocytic and animal models in the study of protein phosphorylation

Luciana Bordin, Micahel D. Coleman, Giulio Clari*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Phosphorylation processes are common post-transductional mechanisms, by which it is possible to modulate a number of metabolic pathways. Proteins are highly sensitive to phosphorylation, which governs many protein-protein interactions. The enzymatic activity of some protein tyrosine-kinases is under tyrosine-phosphorylation control, as well as several transmembrane anion-fluxes and cation exchanges. In addition, phosphorylation reactions are involved in intra and extra-cellular 'cross-talk' processes. Early studies adopted laboratory animals to study these little known phosphorylation processes. The main difficulty encountered with these animal techniques was obtaining sufficient kinase or phosphatase activity suitable for studying the enzymatic process. Large amounts of biological material from organs, such as the liver and spleen were necessary to conduct such work with protein kinases. Subsequent studies revealed the ubiquity and complexity of phosphorylation processes and techniques evolved from early rat studies to the adaptation of more rewarding in vitro models. These involved human erythrocytes, which are a convenient source both for the enzymes, we investigated and for their substrates. This preliminary work facilitated the development of more advanced phosphorylative models that are based on cell lines. © 2005 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalEnvironmental Toxicology and Pharmacology
Volume21
Issue number2
Early online date15 Aug 2005
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Phosphorylation
Animals
Animal Models
Proteins
Laboratory Animals
Metabolic Networks and Pathways
Phosphoric Monoester Hydrolases
Liver
Protein-Tyrosine Kinases
Biological materials
Protein Kinases
Anions
Tyrosine
Cations
Rats
Phosphotransferases
Spleen
Erythrocytes
Cells
Fluxes

Keywords

  • human erythrocytes
  • nephrolithiasis
  • phosphorylation
  • rat liver mitochondria

Cite this

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The use of erythrocytic and animal models in the study of protein phosphorylation. / Bordin, Luciana; Coleman, Micahel D.; Clari, Giulio.

In: Environmental Toxicology and Pharmacology, Vol. 21, No. 2, 02.2006, p. 148-152.

Research output: Contribution to journalArticle

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