The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium

Thomas Wassmer, Marine Froissard, Helmut Plattner, Roland Kissmehl, Jean Cohen

Research output: Contribution to journalArticle

Abstract

The vacuolar proton-ATPase (V-ATPase) is a multisubunit enzyme complex that is able to transfer protons over membranes against an electrochemical potential under ATP hydrolysis. The enzyme consists of two subcomplexes: V0, which is membrane embedded; and V1, which is cytosolic. V0 was also reported to be involved in fusion of vacuoles in yeast. We identified six genes encoding c-subunits (proteolipids) of V0 and two genes encoding F-subunits of V1 and studied the role of the V-ATPase in trafficking in Paramecium. Green fluorescent protein (GFP) fusion proteins allowed a clear subcellular localization of c- and F-subunits in the contractile vacuole complex of the osmoregulatory system and in food vacuoles. Several other organelles were also detected, in particular dense core secretory granules (trichocysts). The functional significance of the V-ATPase in Paramecium was investigated by RNA interference (RNAi), using a recently developed feeding method. A novel strategy was used to block the expression of all six c- or both F-subunits simultaneously. The V-ATPase was found to be crucial for osmoregulation, the phagocytotic pathway and the biogenesis of dense core secretory granules. No evidence was found supporting participation of V0 in membrane fusion.
Original languageEnglish
Pages (from-to)2813-2825
Number of pages13
JournalJournal of Cell Science
Volume118
Issue number13
DOIs
Publication statusPublished - 1 Jul 2005

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Paramecium
Vacuolar Proton-Translocating ATPases
Organelles
Protons
Vacuoles
Membranes
Secretory Vesicles
Feeding Methods
Proteolipids
Osmoregulation
Membrane Fusion
Enzymes
RNA Interference
Green Fluorescent Proteins
Genes
Hydrolysis
Adenosine Triphosphate
Yeasts
Food
Proteins

Bibliographical note

© The Company of Biologists Limited 2005. Non-commercial use only

Keywords

  • amino acid sequence
  • animals
  • cloning, molecular
  • gene silencing
  • green fluorescent proteins
  • intracellular membranes
  • mice
  • molecular sequence data
  • molecular weight
  • organelles
  • paramecium
  • RNA interference
  • recombinant fusion proteins
  • saccharomyces cerevisiae
  • sequence alignment
  • vacuolar proton-translocating ATPases
  • vacuoles

Cite this

Wassmer, Thomas ; Froissard, Marine ; Plattner, Helmut ; Kissmehl, Roland ; Cohen, Jean. / The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium. In: Journal of Cell Science. 2005 ; Vol. 118, No. 13. pp. 2813-2825.
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Wassmer, T, Froissard, M, Plattner, H, Kissmehl, R & Cohen, J 2005, 'The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium', Journal of Cell Science, vol. 118, no. 13, pp. 2813-2825. https://doi.org/10.1242/jcs.02405

The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium. / Wassmer, Thomas; Froissard, Marine; Plattner, Helmut; Kissmehl, Roland; Cohen, Jean.

In: Journal of Cell Science, Vol. 118, No. 13, 01.07.2005, p. 2813-2825.

Research output: Contribution to journalArticle

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AU - Wassmer, Thomas

AU - Froissard, Marine

AU - Plattner, Helmut

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AU - Cohen, Jean

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PY - 2005/7/1

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KW - RNA interference

KW - recombinant fusion proteins

KW - saccharomyces cerevisiae

KW - sequence alignment

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