The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

David Y.S. Chau, Naing L. Tint, Russell J Collighan, Martin Griffin, Harminder S. Dua, Kevin M. Shakesheff, Felicity R.A.J. Rose

Research output: Contribution to journalArticle

Abstract

AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.
LanguageEnglish
Pages648-653
Number of pages6
JournalBritish Journal of Ophthalmology
Volume94
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Triamcinolone
Suspensions
Transglutaminases
Vitrectomy
Swine
Corneal Endothelium
Triamcinolone Acetonide
Adhesiveness
Emulsions
Particle Size
Adhesives
Electron Scanning Microscopy
Adsorption
polylactic acid-polyglycolic acid copolymer
Pharmacology
Proteins
In Vitro Techniques

Bibliographical note

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.

Keywords

  • Adhesiveness
  • Glycolates
  • Humans
  • Lactic Acid
  • Microscopy, Electron, Scanning
  • Microspheres
  • Particle Size
  • Polyglycolic Acid
  • Triamcinolone
  • Vitrectomy
  • Vitreous Body

Cite this

Chau, David Y.S. ; Tint, Naing L. ; Collighan, Russell J ; Griffin, Martin ; Dua, Harminder S. ; Shakesheff, Kevin M. ; Rose, Felicity R.A.J. / The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles. In: British Journal of Ophthalmology. 2010 ; Vol. 94, No. 5. pp. 648-653.
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The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles. / Chau, David Y.S.; Tint, Naing L.; Collighan, Russell J; Griffin, Martin; Dua, Harminder S.; Shakesheff, Kevin M.; Rose, Felicity R.A.J.

In: British Journal of Ophthalmology, Vol. 94, No. 5, 2010, p. 648-653.

Research output: Contribution to journalArticle

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T1 - The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

AU - Chau, David Y.S.

AU - Tint, Naing L.

AU - Collighan, Russell J

AU - Griffin, Martin

AU - Dua, Harminder S.

AU - Shakesheff, Kevin M.

AU - Rose, Felicity R.A.J.

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PY - 2010

Y1 - 2010

N2 - AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

AB - AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

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