Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease

Ana Reis , Alisa Rudnitskaya, Pajaree Chariyavilaskul, Neeraj Dhaun, Vanessa Melville, Jane Goddard, David J. Webb, Andrew R. Pitt, Corinne M. Spickett

Research output: Contribution to journalArticle

Abstract

This study compared the molecular lipidomic profi le of LDL in patients with nondiabetic advanced renal disease and no evidence of CVD to that of age-matched controls, with the hypothesis that it would reveal proatherogenic lipid alterations. LDL was isolated from 10 normocholesterolemic patients with stage 4/5 renal disease and 10 controls, and lipids were analyzed by accurate mass LC/MS. Top-down lipidomics analysis and manual examination of the data identifi ed 352 lipid species, and automated comparative analysis demonstrated alterations in lipid profi le in disease. The total lipid and cholesterol content was unchanged, but levels of triacylglycerides and N -acyltaurines were signifi cantly increased, while phosphatidylcholines, plasmenyl ethanolamines, sulfatides, ceramides, and cholesterol sulfate were signifi cantly decreased in chronic kidney disease (CKD) patients. Chemometric analysis of individual lipid species showed very good discrimination of control and disease sample despite the small cohorts and identifi ed individual unsaturated phospholipids and triglycerides mainly responsible for the discrimination. These fi ndings illustrate the point that although the clinical biochemistry parameters may not appear abnormal, there may be important underlying lipidomic changes that contribute to disease pathology. The lipidomic profi le of CKD LDL offers potential for new biomarkers and novel insights into lipid metabolism and cardiovascular risk in this disease. -Reis, A., A. Rudnitskaya, P. Chariyavilaskul, N. Dhaun, V. Melville, J. Goddard, D. J. Webb, A. R. Pitt, and C. M. Spickett. Topdown lipidomics of low density lipoprotein reveal altered lipid profi les in advanced chronic kidney disease. J. Lipid Res. 2015.

LanguageEnglish
Pages413-422
Number of pages10
JournalJournal of Lipid Research
Volume56
Issue number2
Early online date25 Jan 2014
DOIs
Publication statusPublished - 1 Feb 2015

Fingerprint

Chronic Renal Insufficiency
LDL Lipoproteins
Lipids
Ethanolamines
Sulfoglycosphingolipids
Kidney
Ceramides
Biochemistry
Phosphatidylcholines
Lipid Metabolism
Biomarkers
Pathology
Phospholipids
Triglycerides
Chemical vapor deposition
Cholesterol

Bibliographical note

This research was originally published in Journal of Lipid Research. Reis, A., Rudnitskaya, A., Chariyavilaskul, P., Dhaun, N., Melville, V., Goddard, J., ... Spickett, C. M.Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease. Journal of Lipid Research. 2015; 56:413-422. © the American Society for Biochemistry and Molecular Biology.

Keywords

  • Cholesterol
  • Cholesterol sulfate
  • Dyslipidemias
  • Inflammation
  • Mass spectrometry
  • N -acyltaurine
  • Partial least squares discriminant analysis
  • Phospholipids

Cite this

Reis , Ana ; Rudnitskaya, Alisa ; Chariyavilaskul, Pajaree ; Dhaun, Neeraj ; Melville, Vanessa ; Goddard, Jane ; Webb, David J. ; Pitt, Andrew R. ; Spickett, Corinne M. / Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease. In: Journal of Lipid Research. 2015 ; Vol. 56, No. 2. pp. 413-422.
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note = "This research was originally published in Journal of Lipid Research. Reis, A., Rudnitskaya, A., Chariyavilaskul, P., Dhaun, N., Melville, V., Goddard, J., ... Spickett, C. M.Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease. Journal of Lipid Research. 2015; 56:413-422. {\circledC} the American Society for Biochemistry and Molecular Biology.",
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Top-down lipidomics of low density lipoprotein reveal altered lipid profiles in advanced chronic kidney disease. / Reis , Ana; Rudnitskaya, Alisa; Chariyavilaskul, Pajaree; Dhaun, Neeraj; Melville, Vanessa; Goddard, Jane; Webb, David J.; Pitt, Andrew R.; Spickett, Corinne M.

In: Journal of Lipid Research, Vol. 56, No. 2, 01.02.2015, p. 413-422.

Research output: Contribution to journalArticle

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AU - Rudnitskaya, Alisa

AU - Chariyavilaskul, Pajaree

AU - Dhaun, Neeraj

AU - Melville, Vanessa

AU - Goddard, Jane

AU - Webb, David J.

AU - Pitt, Andrew R.

AU - Spickett, Corinne M.

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KW - Cholesterol sulfate

KW - Dyslipidemias

KW - Inflammation

KW - Mass spectrometry

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KW - Partial least squares discriminant analysis

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