A series of fluorescent molecularly imprinted polymers has been prepared with a view to generating material capable of mimicking the binding characteristics of the metabolically important cytochrome isoform CYP2D6. Such polymers would have the possibility to form the sensing element in a high-throughput assay for the prediction of CYP2D6 affinity. The imprinted polymers possessed binding-dependent fluorescence. They re-bound their templates and various cross-reactivities were encountered for test compound/drug recognition. One polymer in particular exhibited a rational discrimination amongst the related synthetic templates and was reasonably successful in recognising CYP2D6 substrates from a drug panel. © 2005 Elsevier B.V. All rights reserved.
Bibliographical noteNOTICE: this is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Rathbone, Daniel L.; Ali, Aisha; Antonaki, Polyxeni and Cheek, Sarah (2005). Towards a polymeric binding mimic for cytochrome CYP2D6. Biosensors and Bioelectronics, 20 (11), pp. 2353-2363. DOI 10.1016/j.bios.2005.01.009
- molecularly imprinted polymer
- binding mimic