Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae

K. S. Pikula, A. M. Zakharenko, V. V. Chaika, A. K. Stratidakis, M. Kokkinakis, G. Waissi, V. N. Rakitskii, D. A. Sarigiannis, A. W. Hayes, M. D. Coleman, A. Tsatsakis, K. S. Golokhvast*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The world biodiesel production is increasing at a rapid rate. Despite its perceived safety for the environment, more detailed toxicity studies are mandatory, especially in the field of aquatic toxicology. While considerable attention has been paid to biodiesel combustion emissions, the toxicity of biodiesel in the aquatic environment has been poorly understood. In our study, we used an algae culture growth-inhibition test (OECD 201) for the comparison of the toxicity of B100 (pure biodiesel), produced by methanol transesterification of waste cooking oil (yellow grease), B0 (petroleum diesel fuel) and B20 (diesel-biodiesel blended of 20% biodiesel and 80% petroleum diesel fuel by volume). Two marine diatoms Attheya ussuriensis and Chaetoceros muelleri, the red algae Porphyridium purpureum and Raphidophyte Heterosigma akashiwo were employed as the aquatic test organisms. A sample of biodiesel from waste cooking oil without dilution with petroleum diesel (B100) showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo, compared to hexane, methanol, petroleum diesel (B0) and diluted sample (B20). The acute EC50 in the growth-inhibition test (96 h exposure) of B100 for the four species was in the range of 3.75–23.95 g/L whereas the chronic toxicity EC50 (7d exposure) was in the range of 0.42–16.09 g/L.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalToxicology Reports
Volume6
Early online date29 Dec 2018
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Microalgae
Biofuels
Bioassay
Cooking
Biological Assay
Toxicity
Oils
Petroleum
Gasoline
Algae
Diesel fuels
Methanol
Porphyridium
Rhodophyta
Diatoms
Aquatic Organisms
Transesterification
Lubricating greases
Hexanes
Growth

Bibliographical note

© 2018 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)

Keywords

  • Aquatic pollution
  • Biodiesel
  • Biodiesel blends
  • Ecotoxicology
  • Microalgae
  • Waste cooking oil biodiesel

Cite this

Pikula, K. S., Zakharenko, A. M., Chaika, V. V., Stratidakis, A. K., Kokkinakis, M., Waissi, G., ... Golokhvast, K. S. (2019). Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae. Toxicology Reports, 6, 111-117. https://doi.org/10.1016/j.toxrep.2018.12.007
Pikula, K. S. ; Zakharenko, A. M. ; Chaika, V. V. ; Stratidakis, A. K. ; Kokkinakis, M. ; Waissi, G. ; Rakitskii, V. N. ; Sarigiannis, D. A. ; Hayes, A. W. ; Coleman, M. D. ; Tsatsakis, A. ; Golokhvast, K. S. / Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae. In: Toxicology Reports. 2019 ; Vol. 6. pp. 111-117.
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abstract = "The world biodiesel production is increasing at a rapid rate. Despite its perceived safety for the environment, more detailed toxicity studies are mandatory, especially in the field of aquatic toxicology. While considerable attention has been paid to biodiesel combustion emissions, the toxicity of biodiesel in the aquatic environment has been poorly understood. In our study, we used an algae culture growth-inhibition test (OECD 201) for the comparison of the toxicity of B100 (pure biodiesel), produced by methanol transesterification of waste cooking oil (yellow grease), B0 (petroleum diesel fuel) and B20 (diesel-biodiesel blended of 20{\%} biodiesel and 80{\%} petroleum diesel fuel by volume). Two marine diatoms Attheya ussuriensis and Chaetoceros muelleri, the red algae Porphyridium purpureum and Raphidophyte Heterosigma akashiwo were employed as the aquatic test organisms. A sample of biodiesel from waste cooking oil without dilution with petroleum diesel (B100) showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo, compared to hexane, methanol, petroleum diesel (B0) and diluted sample (B20). The acute EC50 in the growth-inhibition test (96 h exposure) of B100 for the four species was in the range of 3.75–23.95 g/L whereas the chronic toxicity EC50 (7d exposure) was in the range of 0.42–16.09 g/L.",
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Pikula, KS, Zakharenko, AM, Chaika, VV, Stratidakis, AK, Kokkinakis, M, Waissi, G, Rakitskii, VN, Sarigiannis, DA, Hayes, AW, Coleman, MD, Tsatsakis, A & Golokhvast, KS 2019, 'Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae', Toxicology Reports, vol. 6, pp. 111-117. https://doi.org/10.1016/j.toxrep.2018.12.007

Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae. / Pikula, K. S.; Zakharenko, A. M.; Chaika, V. V.; Stratidakis, A. K.; Kokkinakis, M.; Waissi, G.; Rakitskii, V. N.; Sarigiannis, D. A.; Hayes, A. W.; Coleman, M. D.; Tsatsakis, A.; Golokhvast, K. S.

In: Toxicology Reports, Vol. 6, 01.01.2019, p. 111-117.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae

AU - Pikula, K. S.

AU - Zakharenko, A. M.

AU - Chaika, V. V.

AU - Stratidakis, A. K.

AU - Kokkinakis, M.

AU - Waissi, G.

AU - Rakitskii, V. N.

AU - Sarigiannis, D. A.

AU - Hayes, A. W.

AU - Coleman, M. D.

AU - Tsatsakis, A.

AU - Golokhvast, K. S.

N1 - © 2018 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The world biodiesel production is increasing at a rapid rate. Despite its perceived safety for the environment, more detailed toxicity studies are mandatory, especially in the field of aquatic toxicology. While considerable attention has been paid to biodiesel combustion emissions, the toxicity of biodiesel in the aquatic environment has been poorly understood. In our study, we used an algae culture growth-inhibition test (OECD 201) for the comparison of the toxicity of B100 (pure biodiesel), produced by methanol transesterification of waste cooking oil (yellow grease), B0 (petroleum diesel fuel) and B20 (diesel-biodiesel blended of 20% biodiesel and 80% petroleum diesel fuel by volume). Two marine diatoms Attheya ussuriensis and Chaetoceros muelleri, the red algae Porphyridium purpureum and Raphidophyte Heterosigma akashiwo were employed as the aquatic test organisms. A sample of biodiesel from waste cooking oil without dilution with petroleum diesel (B100) showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo, compared to hexane, methanol, petroleum diesel (B0) and diluted sample (B20). The acute EC50 in the growth-inhibition test (96 h exposure) of B100 for the four species was in the range of 3.75–23.95 g/L whereas the chronic toxicity EC50 (7d exposure) was in the range of 0.42–16.09 g/L.

AB - The world biodiesel production is increasing at a rapid rate. Despite its perceived safety for the environment, more detailed toxicity studies are mandatory, especially in the field of aquatic toxicology. While considerable attention has been paid to biodiesel combustion emissions, the toxicity of biodiesel in the aquatic environment has been poorly understood. In our study, we used an algae culture growth-inhibition test (OECD 201) for the comparison of the toxicity of B100 (pure biodiesel), produced by methanol transesterification of waste cooking oil (yellow grease), B0 (petroleum diesel fuel) and B20 (diesel-biodiesel blended of 20% biodiesel and 80% petroleum diesel fuel by volume). Two marine diatoms Attheya ussuriensis and Chaetoceros muelleri, the red algae Porphyridium purpureum and Raphidophyte Heterosigma akashiwo were employed as the aquatic test organisms. A sample of biodiesel from waste cooking oil without dilution with petroleum diesel (B100) showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo, compared to hexane, methanol, petroleum diesel (B0) and diluted sample (B20). The acute EC50 in the growth-inhibition test (96 h exposure) of B100 for the four species was in the range of 3.75–23.95 g/L whereas the chronic toxicity EC50 (7d exposure) was in the range of 0.42–16.09 g/L.

KW - Aquatic pollution

KW - Biodiesel

KW - Biodiesel blends

KW - Ecotoxicology

KW - Microalgae

KW - Waste cooking oil biodiesel

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Pikula KS, Zakharenko AM, Chaika VV, Stratidakis AK, Kokkinakis M, Waissi G et al. Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae. Toxicology Reports. 2019 Jan 1;6:111-117. https://doi.org/10.1016/j.toxrep.2018.12.007