Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae

K. S. Pikula, A. M. Zakharenko, V. V. Chaika, A. K. Stratidakis, M. Kokkinakis, G. Waissi, V. N. Rakitskii, D. A. Sarigiannis, A. W. Hayes, M. D. Coleman, A. Tsatsakis, K. S. Golokhvast*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The world biodiesel production is increasing at a rapid rate. Despite its perceived safety for the environment, more detailed toxicity studies are mandatory, especially in the field of aquatic toxicology. While considerable attention has been paid to biodiesel combustion emissions, the toxicity of biodiesel in the aquatic environment has been poorly understood. In our study, we used an algae culture growth-inhibition test (OECD 201) for the comparison of the toxicity of B100 (pure biodiesel), produced by methanol transesterification of waste cooking oil (yellow grease), B0 (petroleum diesel fuel) and B20 (diesel-biodiesel blended of 20% biodiesel and 80% petroleum diesel fuel by volume). Two marine diatoms Attheya ussuriensis and Chaetoceros muelleri, the red algae Porphyridium purpureum and Raphidophyte Heterosigma akashiwo were employed as the aquatic test organisms. A sample of biodiesel from waste cooking oil without dilution with petroleum diesel (B100) showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo, compared to hexane, methanol, petroleum diesel (B0) and diluted sample (B20). The acute EC50 in the growth-inhibition test (96 h exposure) of B100 for the four species was in the range of 3.75–23.95 g/L whereas the chronic toxicity EC50 (7d exposure) was in the range of 0.42–16.09 g/L.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalToxicology Reports
Early online date29 Dec 2018
Publication statusPublished - 1 Jan 2019

Bibliographical note

© 2018 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (


  • Aquatic pollution
  • Biodiesel
  • Biodiesel blends
  • Ecotoxicology
  • Microalgae
  • Waste cooking oil biodiesel


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