Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue

R. Saborano; E. Shepherd; U.L. Günther; P.F. Lalor

doi:10.1007/978-1-0716-3247-5_13

Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue

R. Saborano
, E. Shepherd
, U.L. Günther
, P.F. Lalor

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.

Original language	English
Title of host publication	Metabolic Reprogramming
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Chapter	13
Pages	167-180
Number of pages	14
ISBN (Electronic)	9781071632475
ISBN (Print)	9781071632468
DOIs	https://doi.org/10.1007/978-1-0716-3247-5_13
Publication status	Published - 1 Jun 2023

Publication series

Name	Methods in Molecular Biology
Volume	2675
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

1D NMR
2D NMR
Human
Liver
Perfusion

Access to Document

10.1007/978-1-0716-3247-5_13

Cite this

@inbook{a537c12c6f2c41b1906704ecceeb6e01,

title = "Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue",

abstract = "Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.",

keywords = "1D NMR, 2D NMR, Human, Liver, Perfusion",

author = "R. Saborano and E. Shepherd and U.L. G{\"u}nther and P.F. Lalor",

year = "2023",

month = jun,

day = "1",

doi = "10.1007/978-1-0716-3247-5\_13",

language = "English",

isbn = "9781071632468",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "167--180",

booktitle = "Metabolic Reprogramming",

address = "United States",

}

TY - CHAP

T1 - Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue

AU - Saborano, R.

AU - Shepherd, E.

AU - Günther, U.L.

AU - Lalor, P.F.

PY - 2023/6/1

Y1 - 2023/6/1

N2 - Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.

AB - Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.

KW - 1D NMR

KW - 2D NMR

KW - Human

KW - Liver

KW - Perfusion

UR - https://link.springer.com/protocol/10.1007/978-1-0716-3247-5_13

UR - https://www.scopus.com/pages/publications/85160727059

U2 - 10.1007/978-1-0716-3247-5_13

DO - 10.1007/978-1-0716-3247-5_13

M3 - Chapter (peer-reviewed)

SN - 9781071632468

T3 - Methods in Molecular Biology

SP - 167

EP - 180

BT - Metabolic Reprogramming

PB - Humana Press

ER -

Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue

Abstract

Publication series

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this