Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology

Stephane R. Gross, Terri G. Kinzy

Research output: Contribution to journalArticlepeer-review

Abstract

The binding of eukaryotic translation elongation factor 1A (eEF1A) to actin is a noncanonical function that may link two distinct cellular processes, cytoskeleton organization and gene expression. Using the yeast Saccharomyces cerevisiae, we have established an in vivo assay that directly identifies specific regions and residues of eEF1A responsible for actin interactions and bundling. Using a unique genetic screen, we isolated a series of eEF1A mutants with reduced actin bundling activity. These mutations alter actin cytoskeleton organization but not translation, indicating that these are separate functions of eEF1A. This demonstrates for the first time a direct consequence of eEF1A on cytoskeletal organization in vivo and the physiological significance of this interaction.
Original languageEnglish
Pages (from-to)772-778
Number of pages7
JournalNature Structural and Molecular Biology
Volume12
DOIs
Publication statusPublished - 2005

Keywords

  • binding
  • eukaryotic translation elongation factor 1A
  • eEF1A
  • actin
  • cytoskeleton organization
  • gene expression
  • yeast
  • Saccharomyces cerevisiae
  • actin bundling activity
  • mutation

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