We investigate the temperature effect on the size and stability of two major blood plasma proteins, human serum albumin and fibrinogen in aqueous NaCl solution. Dynamic Light Scattering measurements were carried out in the physiological temperature range up to 45 degrees C. The analysis of the results provided the temperature dependences of the macromolecular hydrodynamic radius and the potential. For albumin the hydrodynamic radius remained unchanged, while the zeta-potential increased sharply at approximately 40 degrees C. For fibrinogen the radius increased significantly above 45 degrees C and the zeta-potential increased similar to albumin at slightly below 40 degrees C. The dynamics of albumin macromolecule was simulated using classical Molecular Dynamics, which showed no change in the gyration radius, root mean square deviation, and the composition of disulfide and salt bridges, but substantial change in the secondary structure of the protein. We conclude that these changes in the structure and dynamics of the proteins are correlated with the qualitative change of water dynamics at 42 degrees C in the hydration shell of the proteins.
Bibliographical note© 2019 by the author(s); licensee Accademia Peloritana dei Pericolanti (Messina, Italy). This article is an
open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/).
Funding: This work has been supported by the European Union’s Horizon 2020 research and
innovation program under the Marie Sklodowska-Curie Research and Innovation Staff
Exchange, MSCA-RISE-2018, Proposal number: 823922, AMR-TB. The authors are
grateful to the Erasmus + program Key action 1 – Mobility for Learners and Staff – Higher
Education Student and Staff Mobility (Inter-institutional agreement 01.06.2018-31.07.2020
between institutions from Program and Partner Countries, Aston University and Taras
Shevchenko National University of Kiev).
Atamas, N., Bardik, V. Y., Komisarenko, S., Makogonenko, Y. M., Lugovskoi, E. V., Malomuz, N. P., Nerukh, D., & Solonin, P. K. (2019). Water dynamics and stability of major blood proteins at pre-denaturation stage. Atti della Accademia Peloritana dei Pericolanti, 97(Suppl 2), [A16]. https://doi.org/10.1478/AAPP.97S2A16