Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review)

Richard A.J. Darby; Stephanie P. Cartwright; Marvin V. Dilworth; Roslyn M. Bill

doi:10.1007/978-1-61779-770-5_2

Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review)

Richard A.J. Darby
, Stephanie P. Cartwright
, Marvin V. Dilworth
, Roslyn M. Bill

John Radcliffe Hospital
University of Oxford

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

61 Citations (Scopus)

2033 Downloads (Pure)

Abstract

Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species of yeast to date, this could pose a significant challenge. However, in reality, only very few species of yeast have been employed as host organisms for the production of recombinant proteins. The two most widely used, Saccharomyces cerevisiae and Pichia pastoris, are compared and contrasted here.

Original language	English
Title of host publication	Recombinant protein production in yeast
Subtitle of host publication	methods and protocols
Editors	Roslyn M. Bill
Publisher	Humana Press
Pages	11-23
Number of pages	13
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5_2
Publication status	Published - 25 Feb 2012

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Bibliographical note

The original publication is available at www.springerlink.com

Keywords

yeast
host cell
recombinant protein production

Access to Document

10.1007/978-1-61779-770-5_2

Which yeast species shall I choose
The original publication is available at www.springerlink.com
Accepted author manuscript, 139 KB

http://www.springerlink.com/content/j451t7g703878677/#section=1054613&page=1

Cite this

Darby, R. A. J., Cartwright, S. P., Dilworth, M. V., & Bill, R. M. (2012). Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review). In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 11-23). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_2

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Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review). / Darby, Richard A.J.; Cartwright, Stephanie P.; Dilworth, Marvin V. et al.
Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 11-23 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

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Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review)

Abstract

Publication series

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Large-scale production of secreted proteins in Pichia pastoris

Optimising Pichia pastoris induction

Optimising yeast as a host for recombinant protein production (review)

Cite this