Zinc-α2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia

Chen Bing, Yi Bao, John Jenkins, Paul Sanders, Monia Manieri, Saverio Cinti, Michael J. Tisdale, Paul Trayhurn*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Zinc-α2-glycoprotein (ZAG), a 43-kDa protein, is overexpressed in certain human malignant tumors and acts as a lipid-mobilizing factor to stimulate lipolysis in adipocytes leading to cachexia in mice implanted with ZAG-producing tumors. Because white adipose tissue (WAT) is an endocrine organ secreting a wide range of protein factors, including those involved in lipid metabolism, we have investigated whether ZAG is produced locally by adipocytes. ZAG mRNA was detected by RT-PCR in the mouse WAT depots examined (epididymal, perirenal, s.c., and mammary gland) and in interscapular brown fat. In WAT, ZAG gene expression was evident in mature adipocytes and in stromal-vascular cells. Using a ZAG Ab, ZAG protein was located in WAT by Western blotting and immunohistochemistry. Mice bearing the MAC16-tumor displayed substantial losses of body weight and fat mass, which was accompanied by major increases in ZAG mRNA and protein levels in WAT and brown fat. ZAG mRNA was detected in 3T3-L1 cells, before and after the induction of differentiation, with the level increasing progressively after differentiation with a peak at days 8-10. Both dexamethasone and a β 3 agonist, BRL 37344, increased ZAG mRNA levels in 3T3-L1 adipocytes. ZAG gene expression and protein were also detected in human adipose tissue (visceral and s.c.). It is suggested that ZAG is a new adipose tissue protein factor, which may be involved in the modulation of lipolysis in adipocytes. Overexpression in WAT of tumor-bearing mice suggests a local role for adipocyte-derived ZAG in the substantial reduction of adiposity of cancer cachexia.

Original languageEnglish
Pages (from-to)2500-2505
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume101
Issue number8
DOIs
Publication statusPublished - 24 Feb 2004

Fingerprint

White Adipose Tissue
Cachexia
Adipocytes
Zinc
Glycoproteins
Neoplasms
Messenger RNA
Proteins
Brown Adipose Tissue
Lipolysis
Adipose Tissue
3T3-L1 Cells
Gene Expression
Intra-Abdominal Fat
Adiposity
Thromboplastin
Human Mammary Glands
Stromal Cells
Lipid Metabolism
Dexamethasone

Keywords

  • Zinc-alpha2-glycoprotein (ZAG)
  • 43-kDa protein
  • human malignant tumors
  • lipid-mobilizing factor
  • lipolysis
  • adipocyte
  • cachexia
  • white adipose tissue
  • lipid metabolism
  • cancer cachexia

Cite this

Bing, Chen ; Bao, Yi ; Jenkins, John ; Sanders, Paul ; Manieri, Monia ; Cinti, Saverio ; Tisdale, Michael J. ; Trayhurn, Paul. / Zinc-α2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia. In: Proceedings of the National Academy of Sciences. 2004 ; Vol. 101, No. 8. pp. 2500-2505.
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Zinc-α2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia. / Bing, Chen; Bao, Yi; Jenkins, John; Sanders, Paul; Manieri, Monia; Cinti, Saverio; Tisdale, Michael J.; Trayhurn, Paul.

In: Proceedings of the National Academy of Sciences, Vol. 101, No. 8, 24.02.2004, p. 2500-2505.

Research output: Contribution to journalArticle

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AU - Bing, Chen

AU - Bao, Yi

AU - Jenkins, John

AU - Sanders, Paul

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AB - Zinc-α2-glycoprotein (ZAG), a 43-kDa protein, is overexpressed in certain human malignant tumors and acts as a lipid-mobilizing factor to stimulate lipolysis in adipocytes leading to cachexia in mice implanted with ZAG-producing tumors. Because white adipose tissue (WAT) is an endocrine organ secreting a wide range of protein factors, including those involved in lipid metabolism, we have investigated whether ZAG is produced locally by adipocytes. ZAG mRNA was detected by RT-PCR in the mouse WAT depots examined (epididymal, perirenal, s.c., and mammary gland) and in interscapular brown fat. In WAT, ZAG gene expression was evident in mature adipocytes and in stromal-vascular cells. Using a ZAG Ab, ZAG protein was located in WAT by Western blotting and immunohistochemistry. Mice bearing the MAC16-tumor displayed substantial losses of body weight and fat mass, which was accompanied by major increases in ZAG mRNA and protein levels in WAT and brown fat. ZAG mRNA was detected in 3T3-L1 cells, before and after the induction of differentiation, with the level increasing progressively after differentiation with a peak at days 8-10. Both dexamethasone and a β 3 agonist, BRL 37344, increased ZAG mRNA levels in 3T3-L1 adipocytes. ZAG gene expression and protein were also detected in human adipose tissue (visceral and s.c.). It is suggested that ZAG is a new adipose tissue protein factor, which may be involved in the modulation of lipolysis in adipocytes. Overexpression in WAT of tumor-bearing mice suggests a local role for adipocyte-derived ZAG in the substantial reduction of adiposity of cancer cachexia.

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